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Home Biochemical Engineering Fermentation Reactors Comments

  • Question
  • In fed batch bioreactor modelling, the rate of change in the bioreactor volume is assumed to be equal to


  • Options
  • A. flow rate
  • B. initial volume
  • C. volume of solids in the reactor
  • D. none of these

  • Correct Answer
  • flow rate 


  • Fermentation Reactors problems


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    • 1. In a plug flow reactor, the highest concentration of substrates is exposed to the cells that

    • Options
    • A. are near the effluent or exit of the reactor
    • B. are in the mid way along the reactor
    • C. are located near entrance of feed
    • D. are near the wall of the reactor
    • Discuss
    • 2. Pseudo-steady state in a continuous reactor refers to a reactor in which

    • Options
    • A. Pseudomonads are being cultured
    • B. concentration oscillate around a mean value
    • C. concentration increase with time
    • D. concentration decrease with time
    • Discuss
    • 3. In the batch-fed mode, substrate will be added

    • Options
    • A. only at the beginning of the fermentation process
    • B. periodically throughout the fermentation process
    • C. only at the end of the fermentation process
    • D. substrate is never added
    • Discuss
    • 4. Which of the following organisms will continue their metabolism at high substrate concentrations?

    • Options
    • A. Pseudomonas aeruginosa
    • B. Saccharomyces cerevisiae
    • C. Bacillus subtilis
    • D. Escherichia coli
    • Discuss
    • 5. Pseudomonas aeruginosa has a maximum specific growth rate of 0.8 h-1 in a medium with glucose as a growth limiting substrate. To prevent wash out, the dilution rate must be set to

    • Options
    • A. greater than 0.8 h-1
    • B. less than 0.8 h-1
    • C. equal to 0.8 h-1
    • D. 1.6 h-1
    • Discuss
    • 6. Which of the following is not correct with regards to continuous cultures?

    • Options
    • A. The major industrial scale application of continuous cultures is in waste water treatment
    • B. Mutation and contamination present a major risk to the operation of continuous cultures for the production of metabolites
    • C. The continuous cultures is very useful for the production of pharmaceuticals
    • D. Continuous cultures have an advantage that they do not need to shut down as regularly as batch culture reactor
    • Discuss
    • 7. Two continuous bioreactors containing the same organisms, fed with the same feed at the same dilution rate were compared. Reactor 1 started with an initial concentration of glucose of 10 g.l-1, while reactor 2 contained 0.1 g.l-1 of glucose at the start of the process then at steady state

    • Options
    • A. the concentration of glucose in reactor 1 would be greater than that in reactor 2
    • B. the concentration of glucose in reactor 1 would be equal to reactor 2
    • C. the concentration of glucose in reactor 1 would always be zero.
    • D. the concentration of glucose in reactor 1 would be less than that in reactor 2
    • Discuss
    • 8. In a glucose-yeast extract medium, Lactococcus lactis has a maximum specific growth rate (um) of 1.23 h-1. What would be the specific growth rate of this organism at steady state in a 4 litre reactor being fed at 2 litres per hour?

    • Options
    • A. 0.5 h-1
    • B. 1.2 h-1
    • C. 2.4 h-1
    • D. 4 h-1
    • Discuss
    • 9. The toxicity of organic acids to cells

    • Options
    • A. enhances as the pH falls
    • B. reduces as the pH falls
    • C. is not affected by pH
    • D. is highest at pH 7.0
    • Discuss
    • 10. A microorganism which is subjected to the Crabtree effect is most likely to

    • Options
    • A. ferment in the presence of low concentrations of sugar and dissolved oxygen
    • B. respire in the presence of low sugar and high dissolved oxygen concentrations
    • C. ferment in the presence of high concentrations of sugar and dissolved oxygen
    • D. respire in the presence of high sugar and low dissolved oxygen concentrations
    • Discuss


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