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- Question
For an enzyme that displays Michaelis-Menten kinetics, the reaction velocity (as a fraction of Vmax) observed at [S] = 2 KM will be
Options- A. 0.09
- B. 0.33
- C. 0.66
- D. 0.91
- Correct Answer
- 0.66
- 1. An enzyme has a Km of 4.7 x 10-5M. If the Vmax of the preparation is 22m moles liter-1 min-1, what velocity would be observed in the presence of 2.0 x 10-4M substrate and 5.0 x 10-5M of a competitive inhibitor?
Options- A. 13.54? moles liter-1min-1
- B. 6.68? moles liter-1min-1
- C. 7.57? moles liter-1min-1
- D. 17.8? moles liter-1min-1 Discuss
- 2. The slope of Lineweaver Burk plot for Michaelis Menten equation is
Options- A. Vmax/Km
- B. Km/Vmax
- C. 1/Km
- D. Km.Vmax Discuss
- 3. Which of the following statement(s) regarding enzymes, is/are false?
Options- A. Enzymes are always proteins that function as catalysts
- B. Enzymes provide activation energy for reactions
- C. Enzyme activity can be regulated
- D. Enzymes may be used many times for a specific reaction Discuss
- 4. The equation for the rate of product formation for simple enzyme reaction is given by (Where rmax, maximum reaction rate, Cs substrate concentration, Cp product concentration ES, CES enzyme-substrate concentration)
Options- A. rp = rmax Cs/(Km+Cs)
- B. rp= rmax CES/(Km+ CES)
- C. rp = rmax Cs/(Km+CES)
- D. rp = rmax Cs/(Km+Cp) Discuss
- 5. In the steady state the material balance equation for any component of a system is
Options- A. rate of addition + rate of removal - rate of formation = 0
- B. rate of addition - rate of removal + rate of formation = 0
- C. rate of addition + rate of removal + rate of formation = 0
- D. none of the above Discuss
- 6. The usual method(s) to solve rate equation of simple enzyme kinetics is/are
Options- A. Michaelis Menten approach
- B. Briggs-Haldane approach
- C. Numerical solution approach
- D. all of these Discuss
- 7. When an enzyme is functioning at Vmax, the rate of the reaction is limited by
Options- A. the number of collisions between enzyme and substrate
- B. the number of substrate molecules in the reaction
- C. the concentration of the substrate
- D. the rate at which the enzyme can convert substrate to product Discuss
- 8. If a reaction occurs in the absence of inhibitor with rate ?0 and in the presence of inhibitor with rate ?i, the degree of inhibition is defined as
Options- A. (?0 - ?i)/?0
- B. (?0 + ?i)/?0
- C. (?0?i)/?0
- D. (?0-?i)/?i Discuss
- 9. Which of the following step is assumed to be the slowest step in the Michaelis Menten equation?
Options- A. The substrate consuming step
- B. The product releasing step
- C. Formation of enzyme substrate complex
- D. None of these Discuss
- 10. The ratio of the amount of a protein present in a sample, which is used as a measure of purification, is known as
Options- A. specific activity
- B. relative activity
- C. purity ratio
- D. all of these Discuss
Enzymes and Kinetics problems
Search Results
Correct Answer: 13.54? moles liter-1min-1
Correct Answer: Km/Vmax
Correct Answer: Enzymes provide activation energy for reactions
Correct Answer: rp = rmax Cs/(Km+Cs)
Correct Answer: rate of addition - rate of removal + rate of formation = 0
Correct Answer: all of these
Correct Answer: the rate at which the enzyme can convert substrate to product
Correct Answer: (?0 - ?i)/?0
Correct Answer: The product releasing step
Correct Answer: specific activity
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