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Biochemical Engineering
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Immobilized Enzyme
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Question
Which of the following is considered as a disadvantage to the adsorption method of immobilization?
Options
A. It is possible to separate and purify the enzymes while being immobilized
B. The enzymes are not usually deactivated by adsorption
C. The adsorption is a reversible process
D. State of immobilization is very sensitive to solution pH, ionic strength and temperature
Correct Answer
State of immobilization is very sensitive to solution pH, ionic strength and temperature
More questions
1. Which of the following is considered as a disadvantage to the adsorption method of immobilization?
Options
A. It is possible to separate and purify the enzymes while being immobilized
B. The enzymes are not usually deactivated by adsorption
C. The adsorption is a reversible process
D. State of immobilization is very sensitive to solution pH, ionic strength and temperature
Show Answer
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Discuss
Correct Answer: State of immobilization is very sensitive to solution pH, ionic strength and temperature
2. Which of the following statements is not true?
Options
A. Enzymes are proteins that bind to specific substrates and increase the velocity of reactions involving those substrates
B. Enzymes function by overcoming the activation energy barrier of a reaction
C. Enzymes make thermodynamically favorable reactions to proceed; they cannot make unfavorable reactions to occur
D. Enzymes only function when they are in intact cells
Show Answer
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Correct Answer: Enzymes only function when they are in intact cells
3. A viscoelastic fluid is one in which the viscosity
Options
A. is constant regardless of the stirrer speed or mixing time
B. changes during mixing but returns to its original state after mixing
C. increases with increasing stirrer speed
D. decreases with increasing stirrer speed
Show Answer
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Correct Answer: changes during mixing but returns to its original state after mixing
4. An allosteric inhibitor of an enzyme usually
Options
A. participates in feedback regulation
B. denatures the enzyme
C. is a hydrophobic compound
D. causes the enzyme to work faster
Show Answer
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Discuss
Correct Answer: participates in feedback regulation
5. Juice clarification extraction is facilitated by using
Options
A. cellulases
B. amylase
C. inulinase
D. lactase
Show Answer
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Discuss
Correct Answer: cellulases
6. Chemostats work on the principle of
Options
A. maintaining constant volume of culture medium
B. maintaining continuous flow of nutrients
C. maintaining uniform nutrients concentration
D. operating at higher pressure
Show Answer
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Correct Answer: maintaining uniform nutrients concentration
7. In a plug flow reactor, the highest concentration of substrates is exposed to the cells that
Options
A. are near the effluent or exit of the reactor
B. are in the mid way along the reactor
C. are located near entrance of feed
D. are near the wall of the reactor
Show Answer
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Correct Answer: are located near entrance of feed
8. The continuous cultures are not widely used in industry because
Options
A. they are not suited for the production of secondary metabolites
B. contamination or mutation can have a disastrous effect on the operation
C. the government will not approve the licensing of pharmaceuticals produced in continuous cultures
D. all of the above
Show Answer
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Correct Answer: all of the above
9. Transient flow range of mixing speed is obtained for
Options
A.
Re
i
<10
B. 10<
Re
i
<10
4
C.
Re
i
>10
4
D. None of these
Show Answer
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Discuss
Correct Answer: 10<
Re
i
<10
4
10. Henry's law relates
Options
A. the partial pressure of oxygen and the saturation concentration of oxygen in the liquid
B. the oxygen transfer rate and the bubble size
C. the oxygen transfer rate and the temperature
D. the oxygen transfer rate to the partial pressure of oxygen in the liquid
Show Answer
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Correct Answer: the partial pressure of oxygen and the saturation concentration of oxygen in the liquid
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Enzymes and Application
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Immobilized Enzyme
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