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Home Biochemical Engineering Immobilized Enzyme Comments

  • Question
  • Which of the following is considered as a disadvantage to the adsorption method of immobilization?


  • Options
  • A. It is possible to separate and purify the enzymes while being immobilized
  • B. The enzymes are not usually deactivated by adsorption
  • C. The adsorption is a reversible process
  • D. State of immobilization is very sensitive to solution pH, ionic strength and temperature

  • Correct Answer
  • State of immobilization is very sensitive to solution pH, ionic strength and temperature 


  • Immobilized Enzyme problems


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    • 1. The most commonly employed cross-linked polymer is the

    • Options
    • A. polyacrylamide gel
    • B. collagen
    • C. celluloses
    • D. cation exchange resin
    • Discuss
    • 2. The effectiveness factor increases with the

    • Options
    • A. increase of diffusivity and decreased with the increase of particle size
    • B. increase of diffusivity and increased with the increase of particle size
    • C. decrease of diffusivity and decreased with the increase of particle size
    • D. increase of diffusivity and decrease with the decrease of particle size
    • Discuss
    • 3. For glucose isomerization by immobilized enzyme, the reactor generally used is

    • Options
    • A. CSTR
    • B. plug flow
    • C. packed bed
    • D. fluidized bed
    • Discuss
    • 4. The covalent attachment of enzyme molecules is via

    • Options
    • A. nonessential amino acids residues to water insoluble, functional supports
    • B. essential amino acids residues to water insoluble, functional supports
    • C. nonessential amino acids residues to water soluble, functional supports
    • D. essential amino acids residues to water soluble, functional supports
    • Discuss
    • 5. Which of the following is taken as an assumption in the distributed model?

    • Options
    • A. The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme.
    • B. Mass transfer through the immobilized enzyme occurs via molecular diffusion
    • C. There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme
    • D. All of the above
    • Discuss
    • 6. The immobilized enzyme produced by micro encapsulation technique provides

    • Options
    • A. an extremely large surface area
    • B. smaller surface area
    • C. high amount of solvent
    • D. relatively smaller surface area
    • Discuss
    • 7. Which of the following is the commonly employed adsorbents?

    • Options
    • A. Calcium carbonate
    • B. Alumina
    • C. Celluloses
    • D. All of these
    • Discuss
    • 8. Various techniques are available for determining the effective diffusivity of solute in gel. Thin disk method uses

    • Options
    • A. a diffusion cell with two compartments divided by a thin gel
    • B. a diffusion cell with two compartments
    • C. a diffusion cell with two compartments plus by a thin gel
    • D. a diffusion cell with two compartments multiply by a thin gel
    • Discuss
    • 9. Which medium is used for the production of Penicillin using immobilized cells

    • Options
    • A. 1% peptone medium
    • B. glucose medium
    • C. Yeast extract medium
    • D. LB broth
    • Discuss
    • 10. Which of the following is not a physical method of immobilization?

    • Options
    • A. Adsorption
    • B. Entrapment
    • C. Micro encapsulation
    • D. None of these
    • Discuss


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