Discussion
Home ‣ Biochemical Engineering ‣ Immobilized Enzyme Comments
- Question
The most commonly employed cross-linked polymer is the
Options- A. polyacrylamide gel
- B. collagen
- C. celluloses
- D. cation exchange resin
- Correct Answer
- polyacrylamide gel
- 1. The effectiveness factor increases with the
Options- A. increase of diffusivity and decreased with the increase of particle size
- B. increase of diffusivity and increased with the increase of particle size
- C. decrease of diffusivity and decreased with the increase of particle size
- D. increase of diffusivity and decrease with the decrease of particle size Discuss
- 2. For glucose isomerization by immobilized enzyme, the reactor generally used is
Options- A. CSTR
- B. plug flow
- C. packed bed
- D. fluidized bed Discuss
- 3. The covalent attachment of enzyme molecules is via
Options- A. nonessential amino acids residues to water insoluble, functional supports
- B. essential amino acids residues to water insoluble, functional supports
- C. nonessential amino acids residues to water soluble, functional supports
- D. essential amino acids residues to water soluble, functional supports Discuss
- 4. Which of the following is taken as an assumption in the distributed model?
Options- A. The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme.
- B. Mass transfer through the immobilized enzyme occurs via molecular diffusion
- C. There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme
- D. All of the above Discuss
- 5. Functional groups of the nonessential amino acid residues that are suitable for the immobilization process are
Options- A. free ?-, ?- or ? carb oxyl groups
- B. ? or ? amino groups
- C. phenyl, hydroxyl, sulfhydryl or imidazole groups
- D. all of the above Discuss
- 6. Which of the following is considered as a disadvantage to the adsorption method of immobilization?
Options- A. It is possible to separate and purify the enzymes while being immobilized
- B. The enzymes are not usually deactivated by adsorption
- C. The adsorption is a reversible process
- D. State of immobilization is very sensitive to solution pH, ionic strength and temperature Discuss
- 7. The immobilized enzyme produced by micro encapsulation technique provides
Options- A. an extremely large surface area
- B. smaller surface area
- C. high amount of solvent
- D. relatively smaller surface area Discuss
- 8. Which of the following is the commonly employed adsorbents?
Options- A. Calcium carbonate
- B. Alumina
- C. Celluloses
- D. All of these Discuss
- 9. Various techniques are available for determining the effective diffusivity of solute in gel. Thin disk method uses
Options- A. a diffusion cell with two compartments divided by a thin gel
- B. a diffusion cell with two compartments
- C. a diffusion cell with two compartments plus by a thin gel
- D. a diffusion cell with two compartments multiply by a thin gel Discuss
- 10. Which medium is used for the production of Penicillin using immobilized cells
Options- A. 1% peptone medium
- B. glucose medium
- C. Yeast extract medium
- D. LB broth Discuss
Immobilized Enzyme problems
Search Results
Correct Answer: increase of diffusivity and decreased with the increase of particle size
Correct Answer: packed bed
Correct Answer: nonessential amino acids residues to water insoluble, functional supports
Correct Answer: All of the above
Correct Answer: all of the above
Correct Answer: State of immobilization is very sensitive to solution pH, ionic strength and temperature
Correct Answer: an extremely large surface area
Correct Answer: All of these
Correct Answer: a diffusion cell with two compartments divided by a thin gel
Correct Answer: glucose medium
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