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Biochemical Engineering
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Immobilized Enzyme
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Question
The effectiveness factor increases with the
Options
A. increase of diffusivity and decreased with the increase of particle size
B. increase of diffusivity and increased with the increase of particle size
C. decrease of diffusivity and decreased with the increase of particle size
D. increase of diffusivity and decrease with the decrease of particle size
Correct Answer
increase of diffusivity and decreased with the increase of particle size
Immobilized Enzyme problems
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1. For glucose isomerization by immobilized enzyme, the reactor generally used is
Options
A. CSTR
B. plug flow
C. packed bed
D. fluidized bed
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Correct Answer: packed bed
2. The covalent attachment of enzyme molecules is via
Options
A. nonessential amino acids residues to water insoluble, functional supports
B. essential amino acids residues to water insoluble, functional supports
C. nonessential amino acids residues to water soluble, functional supports
D. essential amino acids residues to water soluble, functional supports
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Correct Answer: nonessential amino acids residues to water insoluble, functional supports
3. Which of the following is taken as an assumption in the distributed model?
Options
A. The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme.
B. Mass transfer through the immobilized enzyme occurs via molecular diffusion
C. There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme
D. All of the above
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Correct Answer: All of the above
4. Functional groups of the nonessential amino acid residues that are suitable for the immobilization process are
Options
A. free ?-, ?- or ? carb oxyl groups
B. ? or ? amino groups
C. phenyl, hydroxyl, sulfhydryl or imidazole groups
D. all of the above
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Correct Answer: all of the above
5. During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is
Options
A. equal to that of substrate consumption
B. more than that of substrate consumption
C. lesser than that of substrate consumption
D. is nothing to do with the substrate consumption
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Correct Answer: equal to that of substrate consumption
6. The most commonly employed cross-linked polymer is the
Options
A. polyacrylamide gel
B. collagen
C. celluloses
D. cation exchange resin
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Correct Answer: polyacrylamide gel
7. Which of the following is considered as a disadvantage to the adsorption method of immobilization?
Options
A. It is possible to separate and purify the enzymes while being immobilized
B. The enzymes are not usually deactivated by adsorption
C. The adsorption is a reversible process
D. State of immobilization is very sensitive to solution pH, ionic strength and temperature
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Correct Answer: State of immobilization is very sensitive to solution pH, ionic strength and temperature
8. The immobilized enzyme produced by micro encapsulation technique provides
Options
A. an extremely large surface area
B. smaller surface area
C. high amount of solvent
D. relatively smaller surface area
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Correct Answer: an extremely large surface area
9. Which of the following is the commonly employed adsorbents?
Options
A. Calcium carbonate
B. Alumina
C. Celluloses
D. All of these
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Correct Answer: All of these
10. Various techniques are available for determining the effective diffusivity of solute in gel. Thin disk method uses
Options
A. a diffusion cell with two compartments divided by a thin gel
B. a diffusion cell with two compartments
C. a diffusion cell with two compartments plus by a thin gel
D. a diffusion cell with two compartments multiply by a thin gel
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Correct Answer: a diffusion cell with two compartments divided by a thin gel
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More in Biochemical Engineering:
Agitation and Aeration
Downstream Processing
Enzymes and Application
Enzymes and Kinetics
Fermentation Kinetics
Fermentation Reactors
Fluid Flow
Immobilized Enzyme
Sterilization
Water Treatment