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Biochemical Engineering
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Immobilized Enzyme
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Question
Functional groups of the nonessential amino acid residues that are suitable for the immobilization process are
Options
A. free ?-, ?- or ? carb oxyl groups
B. ? or ? amino groups
C. phenyl, hydroxyl, sulfhydryl or imidazole groups
D. all of the above
Correct Answer
all of the above
Immobilized Enzyme problems
Search Results
1. During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is
Options
A. equal to that of substrate consumption
B. more than that of substrate consumption
C. lesser than that of substrate consumption
D. is nothing to do with the substrate consumption
Show Answer
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Discuss
Correct Answer: equal to that of substrate consumption
2. The immobilized technique involving chemical method is
Options
A. covalent bond formation dependent
B. non-covalent bond formation dependent
C. both (a) and (b)
D. ionic bond formation dependent
Show Answer
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Correct Answer: covalent bond formation dependent
3. Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have
Options
A. low molecular weight
B. high molecular weight
C. high equivalent weight
D. low reactivity
Show Answer
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Discuss
Correct Answer: low molecular weight
4. The support material for immobilization of cells of
Bacillus subtilis
is
Options
A. ion exchange resins
B. gelatin
C. Anthracite
D. agarose and carbodiimide
Show Answer
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Correct Answer: agarose and carbodiimide
5. The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by
Options
A. copolymerization or microencapsulation
B. crosslinking using multifunctional reagents
C. adsorption
D. all of the above
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Correct Answer: copolymerization or microencapsulation
6. Which of the following is taken as an assumption in the distributed model?
Options
A. The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme.
B. Mass transfer through the immobilized enzyme occurs via molecular diffusion
C. There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme
D. All of the above
Show Answer
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Correct Answer: All of the above
7. The covalent attachment of enzyme molecules is via
Options
A. nonessential amino acids residues to water insoluble, functional supports
B. essential amino acids residues to water insoluble, functional supports
C. nonessential amino acids residues to water soluble, functional supports
D. essential amino acids residues to water soluble, functional supports
Show Answer
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Correct Answer: nonessential amino acids residues to water insoluble, functional supports
8. For glucose isomerization by immobilized enzyme, the reactor generally used is
Options
A. CSTR
B. plug flow
C. packed bed
D. fluidized bed
Show Answer
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Correct Answer: packed bed
9. The effectiveness factor increases with the
Options
A. increase of diffusivity and decreased with the increase of particle size
B. increase of diffusivity and increased with the increase of particle size
C. decrease of diffusivity and decreased with the increase of particle size
D. increase of diffusivity and decrease with the decrease of particle size
Show Answer
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Correct Answer: increase of diffusivity and decreased with the increase of particle size
10. The most commonly employed cross-linked polymer is the
Options
A. polyacrylamide gel
B. collagen
C. celluloses
D. cation exchange resin
Show Answer
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Discuss
Correct Answer: polyacrylamide gel
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Enzymes and Application
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Immobilized Enzyme
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