Discussion
Home ‣ Biochemical Engineering ‣ Immobilized Enzyme Comments
- Question
The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by
Options- A. copolymerization or microencapsulation
- B. crosslinking using multifunctional reagents
- C. adsorption
- D. all of the above
- Correct Answer
- copolymerization or microencapsulation
- 1. Which is not the method for producing immobilized enzymes with multifunctional reagents?
Options- A. Enzymes are adsorbed on the surface active support followed by intermolecular cross linking
- B. Functional groups are introduced on the support to react co-valently with enzymes
- C. Enzymes are cross linked intermolecularly
- D. None of the above Discuss
- 2. The correlation often employed to correlate adsorption data for protein is (where Ymax is the maximum amount of solute adsorbed per mass of adsorbent, X is the mass fraction of solute in the diluent phase in solute-free basis, KL is a constant and Y is the equilibrium value of the mass of solute adsorbed per mass of adsorbent)
Options- A. Y = YmaxX/(KL+X)
- B. Y = Ymax/(KL+X)
- C. Y = YmaxX/KL
- D. Y = Ymax- X/ (KL+X) Discuss
- 3. Which of the following is considered as a pretreatment to the biological feeds?
Options- A. Heating to denature the proteins
- B. Addition of filter aids to increase the porosity
- C. Addition of electrolytes
- D. All of the above Discuss
- 4. Micro filtration refers to the separation of suspended material such as bacteria by using a membrane with spore sizes of
Options- A. 0.02 to 10?m
- B. 1-10A°
- C. 20-30?m
- D. 10-200A° Discuss
- 5. The conventional filtration involves the separation of large particles generally
Options- A. dp>5?m
- B. dp>10?m
- C. dp>15?m
- D. dp>20?m Discuss
- 6. The support material for immobilization of cells of Bacillus subtilis is
Options- A. ion exchange resins
- B. gelatin
- C. Anthracite
- D. agarose and carbodiimide Discuss
- 7. Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have
Options- A. low molecular weight
- B. high molecular weight
- C. high equivalent weight
- D. low reactivity Discuss
- 8. The immobilized technique involving chemical method is
Options- A. covalent bond formation dependent
- B. non-covalent bond formation dependent
- C. both (a) and (b)
- D. ionic bond formation dependent Discuss
- 9. During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is
Options- A. equal to that of substrate consumption
- B. more than that of substrate consumption
- C. lesser than that of substrate consumption
- D. is nothing to do with the substrate consumption Discuss
- 10. Functional groups of the nonessential amino acid residues that are suitable for the immobilization process are
Options- A. free ?-, ?- or ? carb oxyl groups
- B. ? or ? amino groups
- C. phenyl, hydroxyl, sulfhydryl or imidazole groups
- D. all of the above Discuss
Immobilized Enzyme problems
Search Results
Correct Answer: None of the above
Correct Answer: Y = YmaxX/(KL+X)
Correct Answer: All of the above
Correct Answer: 0.02 to 10?m
Correct Answer: dp>10?m
Correct Answer: agarose and carbodiimide
Correct Answer: low molecular weight
Correct Answer: covalent bond formation dependent
Correct Answer: equal to that of substrate consumption
Correct Answer: all of the above
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