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Home Biochemical Engineering Downstream Processing Comments

  • Question
  • The conventional filtration involves the separation of large particles generally


  • Options
  • A. dp>5?m
  • B. dp>10?m
  • C. dp>15?m
  • D. dp>20?m

  • Correct Answer
  • dp>10?m 


  • Downstream Processing problems


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    • 1. If the solute passes through membrane freely, the the rejection coefficient (s) is

    • Options
    • A. 1
    • B. 0
    • C. >1
    • D. <1
    • Discuss
    • 2. Conventional adsorption is a

    • Options
    • A. reversible process
    • B. irreversible process
    • C. either reversible or irreversible
    • D. none of these
    • Discuss
    • 3. The efficiency of cell disruption in a bead mill depends on the

    • Options
    • A. concentration of the cells
    • B. amount and size of beads
    • C. type and rotational speed of agitation
    • D. all of these
    • Discuss
    • 4. For the extractions operation, the selectivity should be

    • Options
    • A. > 1
    • B. < 1
    • C. 1
    • D. zero
    • Discuss
    • 5. For cost analysis in a fermentor system, the depreciation cost varies from

    • Options
    • A. 5-6% of the capital cost
    • B. 6-7% of the capital cost
    • C. 6-10% of the capital cost
    • D. 8-10% of the capital cost
    • Discuss
    • 6. Micro filtration refers to the separation of suspended material such as bacteria by using a membrane with spore sizes of

    • Options
    • A. 0.02 to 10?m
    • B. 1-10A°
    • C. 20-30?m
    • D. 10-200A°
    • Discuss
    • 7. Which of the following is considered as a pretreatment to the biological feeds?

    • Options
    • A. Heating to denature the proteins
    • B. Addition of filter aids to increase the porosity
    • C. Addition of electrolytes
    • D. All of the above
    • Discuss
    • 8. The correlation often employed to correlate adsorption data for protein is (where Ymax is the maximum amount of solute adsorbed per mass of adsorbent, X is the mass fraction of solute in the diluent phase in solute-free basis, KL is a constant and Y is the equilibrium value of the mass of solute adsorbed per mass of adsorbent)

    • Options
    • A. Y = YmaxX/(KL+X)
    • B. Y = Ymax/(KL+X)
    • C. Y = YmaxX/KL
    • D. Y = Ymax- X/ (KL+X)
    • Discuss
    • 9. Which is not the method for producing immobilized enzymes with multifunctional reagents?

    • Options
    • A. Enzymes are adsorbed on the surface active support followed by intermolecular cross linking
    • B. Functional groups are introduced on the support to react co-valently with enzymes
    • C. Enzymes are cross linked intermolecularly
    • D. None of the above
    • Discuss
    • 10. The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by

    • Options
    • A. copolymerization or microencapsulation
    • B. crosslinking using multifunctional reagents
    • C. adsorption
    • D. all of the above
    • Discuss


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