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Home Biochemical Engineering Downstream Processing Comments

  • Question
  • A system which require less solvent and produces a more concentrated extract phase, is desired with a


  • Options
  • A. large distribution coefficients
  • B. small distribution coefficients
  • C. very small distribution coefficients
  • D. constant distribution coefficients

  • Correct Answer
  • large distribution coefficients 


  • Downstream Processing problems


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    • 1. The effectiveness of a solvent can be measured by the

    • Options
    • A. distribution coefficients
    • B. selectivity
    • C. both (a) and (b)
    • D. diffusivity
    • Discuss
    • 2. The optimum wet solid content for the cell suspension for a bead mill is typically somewhere between

    • Options
    • A. 5-15% by volume
    • B. 15-30% by volume
    • C. 30-60% by volume
    • D. 60-90% by volume
    • Discuss
    • 3. Which of the following is not the physical method for the cells rupturing?

    • Options
    • A. Milling
    • B. Homogenization
    • C. Ultrasonication
    • D. Enzymatic digestion
    • Discuss
    • 4. The disk centrifuge is the type of centrifuge used most often for bio separations due to its

    • Options
    • A. continuous operation
    • B. lesser cost
    • C. higher speed
    • D. ease in operation
    • Discuss
    • 5. A typical tubular centrifuge has a bowl of 2 to 5 inch in diameter and 9 to 30 inch in height with a maximum rotating speed of

    • Options
    • A. 15,000 to 50,000 rpm
    • B. 50,000 to 100,000 rpm
    • C. 100,000 to 150,000 rpm
    • D. 150,000 to 200,000 rpm
    • Discuss
    • 6. If the membrane rejects all solute, the rejection coefficient (s) is

    • Options
    • A. 1
    • B. 0
    • C. >1
    • D. <1
    • Discuss
    • 7. Assuming the laminar flow across the filter, the rate of filtration (dVf/dt) can be expressed as a function of pressure drop Dp by the modified D'Arcy's equation as (where A is the area of filtering surface, K is the D'Arcy's filter cake permeability and L is the thickness of the filter cake)

    • Options
    • A. (1/A) dVf/dt = ?p/?(L/K)
    • B. dVf/dt = (1/A)?p/?(L/K)
    • C. (1/A) dVf/dt = ?p/? LK
    • D. dVf/dt = (1/A)?p/? LK
    • Discuss
    • 8. Filtration leaf test results indicate that the filtration rate of a protein product is 100 dry lbs/(ft2 hr). What size production filter would be required to obtain 100 dry lbs of filter cake/hr?

    • Options
    • A. 1.54 ft2
    • B. 3.08 ft2
    • C. 65 ft2
    • D. 65 lbs/ ft2.hr
    • Discuss
    • 9. Which of the operation does not come under upstream processing?

    • Options
    • A. Media preparation
    • B. Inoculum development
    • C. Effluent treatment
    • D. Storage of raw material
    • Discuss
    • 10. An ion exchange resin is composed of

    • Options
    • A. polymeric network
    • B. ionic functional groups
    • C. counter ions
    • D. all of these
    • Discuss


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