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Home Biochemical Engineering Downstream Processing Comments

  • Question
  • The disk centrifuge is the type of centrifuge used most often for bio separations due to its


  • Options
  • A. continuous operation
  • B. lesser cost
  • C. higher speed
  • D. ease in operation

  • Correct Answer
  • lesser cost 


  • Downstream Processing problems


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    • 1. A typical tubular centrifuge has a bowl of 2 to 5 inch in diameter and 9 to 30 inch in height with a maximum rotating speed of

    • Options
    • A. 15,000 to 50,000 rpm
    • B. 50,000 to 100,000 rpm
    • C. 100,000 to 150,000 rpm
    • D. 150,000 to 200,000 rpm
    • Discuss
    • 2. The solvent flux across the membrane can be expressed as (where A is the area, Lp is the membrane permeability, ? is the reflection point, ?p is applied pressure and ?? is the osmotic pressure)

    • Options
    • A. J = Lp(?p - ???)
    • B. J/A = Lp(?p - ???)
    • C. J = Lp/???
    • D. J/A = Lp/?p
    • Discuss
    • 3. Concentration polarization can be reduced further by

    • Options
    • A. pre filtering the solution
    • B. reducing the flow rate per unit membrane surface area
    • C. back washing periodically
    • D. all of the above
    • Discuss
    • 4. Selectivity can be expressed as

    • Options
    • A. ratio of the distribution coefficients in different solute
    • B. mass fraction of the solute in the extract phase at equilibrium
    • C. mass fraction of the solute in the raffinate phase
    • D. ratio of the mass fraction of the solute in the extract phase to the raffinate phase
    • Discuss
    • 5. Start up expenses in a fermentation industry varies from

    • Options
    • A. 0-5% of the capital cost
    • B. 10-15% of the capital cost
    • C. 5-10% of the capital cost
    • D. 15-20% of the capital cost
    • Discuss
    • 6. Which of the following is not the physical method for the cells rupturing?

    • Options
    • A. Milling
    • B. Homogenization
    • C. Ultrasonication
    • D. Enzymatic digestion
    • Discuss
    • 7. The optimum wet solid content for the cell suspension for a bead mill is typically somewhere between

    • Options
    • A. 5-15% by volume
    • B. 15-30% by volume
    • C. 30-60% by volume
    • D. 60-90% by volume
    • Discuss
    • 8. The effectiveness of a solvent can be measured by the

    • Options
    • A. distribution coefficients
    • B. selectivity
    • C. both (a) and (b)
    • D. diffusivity
    • Discuss
    • 9. A system which require less solvent and produces a more concentrated extract phase, is desired with a

    • Options
    • A. large distribution coefficients
    • B. small distribution coefficients
    • C. very small distribution coefficients
    • D. constant distribution coefficients
    • Discuss
    • 10. If the membrane rejects all solute, the rejection coefficient (s) is

    • Options
    • A. 1
    • B. 0
    • C. >1
    • D. <1
    • Discuss


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