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Home Biochemical Engineering Downstream Processing Comments

  • Question
  • Chromatography is based on the


  • Options
  • A. different rate of movement of the solute in the column
  • B. separation of one solute from other constituents by being captured on the adsorbent
  • C. different rate of movement of the solvent in the column
  • D. any of the above

  • Correct Answer
  • different rate of movement of the solute in the column 


  • Downstream Processing problems


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    • 1. In antibiotic manufacturing processes, the fermentation time ranges from

    • Options
    • A. 2-3 weeks
    • B. 1-2 weeks
    • C. 4-5 weeks
    • D. 2-4 weeks
    • Discuss
    • 2. Which of the following can act as a shear protectorant?

    • Options
    • A. Serum
    • B. Silicone oil
    • C. Glucose
    • D. Glutamic acid
    • Discuss
    • 3. A stirred tank reactor containing a fluid with a density of 2.5 g.ml-1 and viscosity of 0.002 kg m-1s-1 is mixed at 120 rpm. If the fluid is mixed with an impeller having diameter 50 cm, then the Reynolds number of the liquid in the reactor is

    • Options
    • A. 2500
    • B. 100
    • C. 12,50,000
    • D. 1,000,000
    • Discuss
    • 4. Which of the following describes the importance of the Kolmogorov eddy size in the biotechnology?

    • Options
    • A. The Kolmogorov eddy size is a measure of the smallest eddy size in a liquid
    • B. When the Kolmogorov eddy size become equivalent or smaller than the cell or particle diameter, then the cell or particle will become susceptible to shear damage
    • C. At the Kolmogorov eddy size, much of the energy of the originating eddy will be released
    • D. All of the above
    • Discuss
    • 5. The approach which is commonly used to determine the level of damage to animal cells in a culture medium is to monitor

    • Options
    • A. the concentration of lactate in the medium
    • B. the level of lactate oxidase activity in the medium
    • C. the level of lactate dehydrogenase activity
    • D. the level of lactase activity in the medium
    • Discuss
    • 6. A common filter medium is the cloth filter generally made of

    • Options
    • A. canvas
    • B. synthetic fabrics
    • C. metal or glass fiber
    • D. all of these
    • Discuss
    • 7. Maintenance cost varies from

    • Options
    • A. 0-4% of the capital cost
    • B. 12-16% of the capital cost
    • C. 4-8% of the capital cost
    • D. 8-12% of the capital cost
    • Discuss
    • 8. The stage wise operation of adsorption is called

    • Options
    • A. contact filtration
    • B. conventional adsorption
    • C. affinity adsorption
    • D. ion exchange
    • Discuss
    • 9. The purity of a solute collected between two times t1 and t2 during chromatographic separation can be calculated as

    • Options
    • A. amount of solute eluted - amount of impurity eluted
    • B. amount of solute eluted / amount of impurity eluted
    • C. amount of solvent eluted + amount of impurity eluted
    • D. amount of solvent eluted / amount of impurity eluted
    • Discuss
    • 10. Start up expenses in a fermentation industry varies from

    • Options
    • A. 0-5% of the capital cost
    • B. 10-15% of the capital cost
    • C. 5-10% of the capital cost
    • D. 15-20% of the capital cost
    • Discuss


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