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Home Biochemical Engineering Fluid Flow Comments

  • Question
  • The approach which is commonly used to determine the level of damage to animal cells in a culture medium is to monitor


  • Options
  • A. the concentration of lactate in the medium
  • B. the level of lactate oxidase activity in the medium
  • C. the level of lactate dehydrogenase activity
  • D. the level of lactase activity in the medium

  • Correct Answer
  • the level of lactate dehydrogenase activity 


  • Fluid Flow problems


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    • 1. The viscosity of a fluid increases with the length of time that it is being mixed. This fluid would be best described as being

    • Options
    • A. newtonian
    • B. rheopectic
    • C. viscoelastic
    • D. thixotropic
    • Discuss
    • 2. Which of the following factors will affect the sensitivity of animal cells to shear conditions?

    • Options
    • A. Cell size
    • B. Availability of nutrients in the medium
    • C. The presence of shear protectorants in the medium
    • D. All of the above
    • Discuss
    • 3. A thixotropic fluid is one in which the viscosity

    • Options
    • A. is constant regardless of the stirrer speed or mixing time
    • B. changes during mixing but returns to its original state after mixing
    • C. increases with mixing time
    • D. decreases with mixing time
    • Discuss
    • 4. The viscosity of water at STP is approximately

    • Options
    • A. 0.005 kg.m-1.s-1
    • B. 0.001 kg.m-1.s-1
    • C. 0.003 kg.m-2.s-1
    • D. 0.1 g.cm-1.s-1
    • Discuss
    • 5. Filamentous fungal cells are more shear sensitive than bacterial cells because of

    • Options
    • A. the presence of citin in cell walls of filamentous fungi
    • B. larger in size than bacterial cells and therefore are more susceptible to shear forces
    • C. fungal cells are much larger than bacterial cells
    • D. all of the above
    • Discuss
    • 6. Which of the following describes the importance of the Kolmogorov eddy size in the biotechnology?

    • Options
    • A. The Kolmogorov eddy size is a measure of the smallest eddy size in a liquid
    • B. When the Kolmogorov eddy size become equivalent or smaller than the cell or particle diameter, then the cell or particle will become susceptible to shear damage
    • C. At the Kolmogorov eddy size, much of the energy of the originating eddy will be released
    • D. All of the above
    • Discuss
    • 7. A stirred tank reactor containing a fluid with a density of 2.5 g.ml-1 and viscosity of 0.002 kg m-1s-1 is mixed at 120 rpm. If the fluid is mixed with an impeller having diameter 50 cm, then the Reynolds number of the liquid in the reactor is

    • Options
    • A. 2500
    • B. 100
    • C. 12,50,000
    • D. 1,000,000
    • Discuss
    • 8. Which of the following can act as a shear protectorant?

    • Options
    • A. Serum
    • B. Silicone oil
    • C. Glucose
    • D. Glutamic acid
    • Discuss
    • 9. In antibiotic manufacturing processes, the fermentation time ranges from

    • Options
    • A. 2-3 weeks
    • B. 1-2 weeks
    • C. 4-5 weeks
    • D. 2-4 weeks
    • Discuss
    • 10. Chromatography is based on the

    • Options
    • A. different rate of movement of the solute in the column
    • B. separation of one solute from other constituents by being captured on the adsorbent
    • C. different rate of movement of the solvent in the column
    • D. any of the above
    • Discuss


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