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Home ‣ Biochemical Engineering ‣ Fermentation Kinetics See What Others Are Saying!
- Question
When intracellular enzymes of whole cells are to be used in a bio-conversion process, it is often necessary to____________the cells.
Options- A. permeabilize
- B. lyophilize
- C. heat-kill
- D. denature
- Correct Answer
- permeabilize
- 1. A Brookfield viscometer cannot determine the shear stress because
Options- A. the shear stress is not defined
- B. a computer is required
- C. the impeller type is given only by numbers
- D. all of the above Discuss
- 2. An enzyme has a Km of 4.7 x 10-5M. If the Vmax of the preparation is 22m moles liter-1 min-1, what velocity would be observed in the presence of 2.0 x 10-4M substrate and 5.0 x 10-5M of a competitive inhibitor?
Options- A. 13.54? moles liter-1min-1
- B. 6.68? moles liter-1min-1
- C. 7.57? moles liter-1min-1
- D. 17.8? moles liter-1min-1 Discuss
- 3. Lock and key theory is based on the compatibility of
Options- A. enzyme and substrate
- B. enzyme and product
- C. enzyme and enzyme substrate complex
- D. enzyme substrate complex and product Discuss
- 4. When the height of an aerated bioreactor is increased, it will increase the oxygen transfer rate by
Options- A. increasing the partial pressure of oxygen at the base of the reactor
- B. decreasing the saturation concentration of oxygen in the reactor
- C. decreasing the gas-holdup
- D. all of the above Discuss
- 5. Which of the following is correct for the off-centre impellers?
Options- A. Off-centre impellers can be used with any size reactor
- B. Off-centre impellers should only be used with reactors with volumes less than 10 m3
- C. The impeller should never be placed in an off-centre position
- D. Off-centre impellers can be used when volume is greater than 10 m3 Discuss
- 6. For turbulent flow, Reynolds number is correlated with power number as (where K, a constant which depends on the container geometry and shape of stirrer; Rei, is Reynolds number for agitator; Di, stirrer diameter and N, stirrer speed)
Options- A. Np = K(Rei)-1
- B. Np = N2Di(Rei)-1
- C. Np = K
- D. Can't be correlated Discuss
- 7. Sanitizer used specifically for vitreous enamel are
Options- A. strong alkalis
- B. strong acids
- C. weak alkali with sodium silicate
- D. none of these Discuss
- 8. The optical density sometimes drops during the lag phase because
Options- A. cells tend to grow without dividing leading to a decrease in the surface area to volume ratio
- B. of fast cell growth causing them to fill up with DNA
- C. cells swell up with water
- D. cells swell up with RNA Discuss
- 9. Turbulent flow range of mixing speed is obtained for
Options- A. Rei<10
- B. 10<Rei<104
- C. Rei>104
- D. None of these Discuss
- 10. In a batch reactor specific growth rate
Options- A. remains constant throughout the process
- B. increases exponentially
- C. changes during the fermentation
- D. is highest during the stationary phase Discuss
More questions
Correct Answer: the shear stress is not defined
Correct Answer: 13.54? moles liter-1min-1
Correct Answer: enzyme and substrate
Correct Answer: increasing the partial pressure of oxygen at the base of the reactor
Correct Answer: Off-centre impellers should only be used with reactors with volumes less than 10 m3
Correct Answer: Np = K
Correct Answer: weak alkali with sodium silicate
Correct Answer: cells tend to grow without dividing leading to a decrease in the surface area to volume ratio
Correct Answer: Rei>104
Correct Answer: changes during the fermentation
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