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Home Biochemical Engineering Fermentation Kinetics Comments

  • Question
  • Why a T-flask used in small-scale cell culture is incubated in a horizontal position?


  • Options
  • A. To save space
  • B. To increase the surface area of the liquid-air interface
  • C. Both (a) and (b)
  • D. To increase the rate of oxygen transfer into the liquid

  • Correct Answer
  • Both (a) and (b) 


  • Fermentation Kinetics problems


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    • 1. The contents in jet loop reactor are mixed through

    • Options
    • A. compressed air
    • B. a mechanical agitator
    • C. external pumping
    • D. any of these
    • Discuss
    • 2. Bacterial growth curve is obtained by plotting

    • Options
    • A. number of cells versus time
    • B. number fo spores versus time
    • C. log of number of cells versus time
    • D. log of number of cells survived versus time
    • Discuss
    • 3. The number of baffles in a standard stirred tank bioreactor is

    • Options
    • A. 8
    • B. 6
    • C. 4
    • D. 2
    • Discuss
    • 4. Heat transfer rates (per unit volume) will be lowest in

    • Options
    • A. Stirred tank bioreactor with biomass recycle
    • B. Continuous air lift bioreactor
    • C. Continuous packed bed reactor
    • D. Continuous fluidized bed bioreactor
    • Discuss
    • 5. Which of the following is correct for the prediction of time requirement in a batch fermenter?

    • Options
    • A. Carrying out experiments
    • B. Mathematical modelling of the fermentation process
    • C. Both (a) and (b)
    • D. It is impossible to predict the time required for fermentation
    • Discuss
    • 6. Wash out in steady state fermentation occurs when

    • Options
    • A. dilution rate is less than maximum specific growth rate
    • B. dilution rate is higher than the maximum specific growth rate
    • C. cell concentration reaches the maximum
    • D. specific growth rate is maximum
    • Discuss
    • 7. Which of the following is not correct for the Monod model and the Michaelis Menten Model?

    • Options
    • A. The Michaelis Menten Model was derived from a curve fitting exercise
    • B. The Michaelis Menten model was derived from an analysis of the mechanism of microbial growth
    • C. The Monod model was derived from an analysis of the mechanism of microbial growth
    • D. All of the above
    • Discuss
    • 8. In the accelerated phase, cell starts to

    • Options
    • A. increase and the division rate increases to reach a maximum
    • B. decrease and the division rate increases to reach a maximum
    • C. increase and the division rate decreases to reach a maximum
    • D. increase and the division rate increases to reach a minimum
    • Discuss
    • 9. An open system in which the growth rate is maintained by adding a nutrient (present in limiting quantities) at the same rate as that medium containing micro-organisms is removed is called

    • Options
    • A. manostat
    • B. chemostat
    • C. turbidostat
    • D. culturostat
    • Discuss
    • 10. The height to diameter ratio (H/D) for the column fermenters is

    • Options
    • A. <3
    • B. >3
    • C. <1.5
    • D. >1.5
    • Discuss


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