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Home Biochemical Engineering Enzymes and Kinetics Comments

  • Question
  • Given an enzyme with a Km = 10m M and Vmax = 100 m mol/min. If [S] = 100 m M, which of the following will be true?


  • Options
  • A. A 10 fold increase in Vmax would increase velocity 10 fold y
  • B. A 10 fold decrease in Km would increase velocity
  • C. Both (a) and (b)
  • D. A 10 fold increase in Vmax would decrease velocity 20 fold

  • Correct Answer
  • A 10 fold increase in Vmax would increase velocity 10 fold y 


  • Enzymes and Kinetics problems


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    • 1. Which category of enzymes belongs to class two in the international classification?

    • Options
    • A. Hydrolases
    • B. Ligases
    • C. Transferases
    • D. Isomerase
    • Discuss
    • 2. The effect of non-competitive inhibition on a Lineweaver-Burk Plot is that

    • Options
    • A. it can move the entire curve to the right
    • B. it can change the y-intercept
    • C. it can change the x-intercept
    • D. all of these
    • Discuss
    • 3. Non-competitive inhibitor of an enzyme catalyzed reaction

    • Options
    • A. decreases Vmax
    • B. binds to Michaelis complex (ES)
    • C. both (a) and (b)
    • D. can actually increase reaction velocity in rare cases
    • Discuss
    • 4. Which of these enzymes contains a Zinc (Zn) ion?

    • Options
    • A. Carboxypeptidase A
    • B. Phosphorylase B kinase
    • C. Tyrosine hydroxylase
    • D. Phosphodiesterase
    • Discuss
    • 5. Which graphical method is used to determine an enzyme degree of cooperativity?

    • Options
    • A. Hill plot
    • B. Koshland curve
    • C. Michaelis-Menten hyperbola
    • D. Can not be determined
    • Discuss
    • 6. Lysozyme

    • Options
    • A. catalyses hydrolysis of ?-1-4 linkages between N-acetyl muranic acid and N-acetyl glucosamine in peptideoglycan
    • B. catalyses hydrolysis of ?-1-4 linkages between N-acetyl muranic acid and N-acetyl glucosamine in peptideoglycan
    • C. catalyses hydrolysis of ?-1-4 linkages between N-diacetyl muranic acid and N-diacetyl glucosamine in peptideoglycan
    • D. all of the above
    • Discuss
    • 7. The reduction in off flavour of beer is practiced through

    • Options
    • A. hersperidinase
    • B. rnase
    • C. invertase
    • D. diacetyl reductase
    • Discuss
    • 8. ?-amylase is an endo enzyme which requires

    • Options
    • A. Ca
    • B. Cu
    • C. Mn
    • D. None of these
    • Discuss
    • 9. Hersperidinase is used for

    • Options
    • A. juice clarification
    • B. juice debittering
    • C. off flavour reduction
    • D. RNA reduction in fish
    • Discuss
    • 10. Enzymes degrade, alter or synthesize a food component through

    • Options
    • A. oxidation/reduction/isomerization
    • B. hydrolysis/synthesis
    • C. group transfer
    • D. all of the above
    • Discuss


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