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- Question
What enzyme forms covalent bonds between restriction fragments?
Options- A. DNA primase
- B. DNA helicase
- C. DNA polymerase
- D. DNA ligase
- Correct Answer
- DNA ligase
- 1. When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ .
Options- A. assortative mating
- B. inbreeding
- C. heterosis
- D. genetic drift Discuss
- 2. Under which of the following conditions would population gene frequencies remain the same?
Options- A. Selection for homozygotes
- B. Small population size
- C. Active migration between groups
- D. Random mating Discuss
- 3. X-rays cause
Options- A. the formation of thymine dimers
- B. ionization of water in the cell
- C. heat
- D. none of the above Discuss
- 4. Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because
Options- A. in both cases molecules migrate to the anode
- B. both techniques rely on a constant charge to mass ratio
- C. both techniques utilize the sieving properties of gels
- D. all of the above Discuss
- 5. The order for the construction of a cDNA fragment from mRNA is to
Options- A. bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
- B. treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
- C. digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
- D. bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase Discuss
- 6. Restriction enzymes
Options- A. protect bacteria from viral infection
- B. cut DNA in a staggered fashion
- C. cut DNAs producing a blunt end
- D. all of the above Discuss
- 7. Which of the following is not true of small inbreeding populations?
Options- A. They tend to loose genetic diversity
- B. There is an increased incidence of recessive diseases
- C. Alleles may become fixed
- D. Mutation is increased Discuss
- 8. A plasmid
Options- A. is a circular DNA molecule
- B. always contains an origin of replication
- C. usually contains one or more restriction sites
- D. all of the above Discuss
- 9. Knockout mice are created by
Options- A. mutagenizing a mouse and selecting for mutant offspring
- B. creating a chimera by fusing cells from two different cell lines
- C. infecting the mouse with a retrovirus
- D. transfecting embryonic stem cells with an altered gene sequence Discuss
- 10. Which of these restriction enzymes produce blunt ends?
Options- A. SaII
- B. EcoRV
- C. XhoI
- D. HindIII Discuss
Recombinant DNA Technology problems
Search Results
Correct Answer: genetic drift
Correct Answer: Random mating
Correct Answer: ionization of water in the cell
Correct Answer: all of the above
Correct Answer: bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
Correct Answer: all of the above
Correct Answer: Mutation is increased
Correct Answer: all of the above
Correct Answer: transfecting embryonic stem cells with an altered gene sequence
Correct Answer: EcoRV
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