Protein Purification Questions

• In ion-exchange chromatography
• Which of the following may be added to stabilize the protein after yeast cells disruption?
• Affinity chromatography deals with the
• A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?
• During successful purification scheme, this may be expected that the
• Proteins separation can be carried out on the basis of
• Gel-filtration chromatography separates on the basis of
• The best way to determine the location of protein in the purification scheme is to measure the