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What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would not bind and be washed away
The OD values would be nearly the same as the assay control
Both (a) and (b)
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

Correct Answer: Both (a) and (b)

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