Question 1

Which of the following statements is true for a refractive index detector in HPLC?

Accepted Answer

It does not respond to many solutes

Question 2

Which can be used as a mobile phase in HPLC applications?

Accepted Answer

Any compound with solubility in liquid

Question 3

Void volume refers to the

Accepted Answer

total volume of eluent in the column the remainder being taken up by the packing material

Question 4

HPLC stands for

Accepted Answer

both (a) and (b)

Question 5

An eluotropic series

Accepted Answer

ranks solvents by their relative abilities to displace solutes from a given absorbent

Question 6

HPLC methods include

Accepted Answer

all of the above

Question 7

In reversed phase HPLC, there is a

Accepted Answer

polar solvent/non-polar column

Question 8

A gradient elution in HPLC is one in which the composition of the solvent

Accepted Answer

is changed continuously or in a series of steps

Question 9

The eluent strength is a measure of

Accepted Answer

solvent adsorption energy

Question 10

Dwell volume is defined as

Accepted Answer

the volume of the column between the point at which solvents are mixed and the beginning of the column

Question 11

Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)?

Accepted Answer

All of the above

Question 12

Column efficiency is measured in terms of number of plates which is

Accepted Answer

inversely related to the square of the peak width

Question 13

An isocratic elution in HPLC is one in which the composition of the solvent

Accepted Answer

remains constant

Question 14

In normal phase HPLC, there is a

Accepted Answer

non polar solvent/polar column

Question 15

For a typical adsorbent such as silica gel, the most popular pore diameters are

Accepted Answer

60 and 100 A°

HPLC Questions