Question 1

In a native PAGE, proteins are separated on the basis of

Accepted Answer

net charge and size

Question 2

In SDS-PAGE, the protein sample is first

Accepted Answer

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

Question 3

Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in

Accepted Answer

histones migrate to the cathode and myoglobin migrates to the anode

Question 4

Proteins are separated in an SDS-PAGE experiment on the basis of their

Accepted Answer

molecular weight

Question 5

In an SDS-PAGE

Accepted Answer

all of the above

Question 6

The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by

Accepted Answer

combining information from (a)and (b)

Question 7

In isoelectric focusing, proteins are separated on the basis of their

Accepted Answer

relative content of positively and negatively charged residue

Question 8

In a gel filtration column

Accepted Answer

both (a) and (b)

Question 9

Proteins can be visualized directly in gels by

Accepted Answer

staining them with the dye

Gel Electrophoresis Questions