FT IR Spectroscopy Questions

• Cytochrome C has an isoelectric pH of
• Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis?
• How do you turn a signal recorded in the time domain into a frequency domain signal?
• Negative staining is a technique used in
• In scanning electron microscopy
• How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field?
• In FT-NMR, how are nuclei excited?
• In immunofluroscence microscopy, fluroscent compounds are attached to
• What is meant by rotating frame of reference?
• The sequence of amino acids in proteins can be determined by means of
• The frequency of precession, the transition frequency and the Larmor frequency are
• What does the Michelson interferometer do?
• When radiation energy is absorbed by a spin 1/2 nucleus in a magnetic field, what happens?
• An FT-IR instrument record a signal in the
• What is the name given to the relaxation process due to an interaction between an excited nucleus and the magnetic fields caused by nuclei in molecules moving around in the sample?
• Why is it advantageous to record many FID signals from the same sample and then add them together?