Whose X-ray diffraction work provided crucial evidence that helped Watson and Crick deduce the DNA double helix?

Difficulty: Easy

Correct Answer: Rosalind Franklin

Explanation:


Introduction / Context:
The double-helix model of DNA proposed by James Watson and Francis Crick in 1953 relied on converging lines of evidence: base-pairing rules, fiber diffraction, and chemical constraints. Understanding whose data underpinned the helix geometry is part of the history of science.


Given Data / Assumptions:

  • X-ray diffraction patterns of DNA fibers constrain helical pitch and symmetry.
  • Contemporaneous biochemical data (e.g., Chargaff’s ratios) limited base pairing possibilities.
  • Multiple scientists contributed to the era’s insights.


Concept / Approach:
Rosalind Franklin’s high-resolution X-ray diffraction photographs (notably Photo 51, taken by her student Raymond Gosling) revealed helical features, spacing of stacked bases (~3.4 Å), and dimensions consistent with a two-strand helix, guiding model building.


Step-by-Step Solution:

Identify whose data directly constrained the helix: Franklin’s fiber diffraction was pivotal.Differentiate historical contributors: Bragg advanced crystallography methods; Pauling proposed alternative structures; Lederberg contributed to bacterial genetics; Chargaff provided base ratios.Select the scientist whose X-ray work was decisive for the helix parameters: Rosalind Franklin.


Verification / Alternative check:
Historical accounts and primary sources credit Franklin’s patterns with helical inference and correct helical parameters used by Watson and Crick.


Why Other Options Are Wrong:

  • W. H. Bragg: Nobel laureate in X-ray crystallography methods, but not the DNA fiber data.
  • Linus Pauling: proposed a triple-helix model that was incorrect for DNA.
  • Joshua Lederberg: genetics pioneer; not responsible for DNA X-ray fiber images.
  • Erwin Chargaff: provided base ratio rules; crucial but not X-ray diffraction.


Common Pitfalls:
Attributing “X-ray evidence” generically to famous names without distinguishing the specific DNA fiber data.


Final Answer:
Rosalind Franklin

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