Difficulty: Easy
Correct Answer: Established cell lines can be maintained in suspension cultures, whereas this is exceptional for primary cell lines
Explanation:
Introduction / Context:
Adherent versus suspension growth is a key practical distinction in cell culture. Many industrial processes prefer suspension-capable lines for scalability in bioreactors. This question distinguishes the adaptability of primary versus established lines to suspension culture.
Given Data / Assumptions:
Concept / Approach:
Most PCLs require attachment (e.g., via extracellular matrix or surface proteins) and exhibit contact inhibition and limited lifespan, making suspension culture unusual. Conversely, many ECLs (e.g., HEK293 derivatives, CHO, hybridomas) can be selected or adapted for suspension growth, enabling large-scale production in stirred-tank bioreactors.
Step-by-Step Solution:
Verification / Alternative check:
Manufacturing platforms (CHO-S, 293-F) demonstrate stable suspension growth and high-density culture, while PCLs (e.g., primary fibroblasts) typically demand adherent culture with serum and matrices.
Why Other Options Are Wrong:
Common Pitfalls:
Assuming any mammalian cell can be trivially adapted to suspension; overlooking the specialized selection needed for some ECLs.
Final Answer:
Established cell lines can be maintained in suspension cultures, whereas this is exceptional for primary cell lines
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