Difficulty: Easy
Correct Answer: All of the above
Explanation:
Introduction / Context:
Chemiosmosis is the unifying concept behind ATP generation in oxidative phosphorylation and photophosphorylation. Understanding the pieces of this mechanism explains how cells convert redox energy into the phosphoanhydride bonds of ATP efficiently and reversibly.
Given Data / Assumptions:
Concept / Approach:
Electrons flow spontaneously down redox potential gradients through membrane protein complexes. The released free energy powers vectorial proton translocation, creating a proton motive force (delta pH and membrane potential). ATP synthase then harnesses proton return to the matrix or stroma, rotating its catalytic head to synthesize ATP. All three listed features are integral to the shared mechanism and therefore the most complete choice is the combined alternative.
Step-by-Step Solution:
Verification / Alternative check:
Experiments with uncouplers collapse the gradient and halt ATP synthesis despite active electron transport, proving the necessity of a proton gradient and ATP synthase coupling.
Why Other Options Are Wrong:
Each single feature is true but incomplete; only the combined answer fully captures chemiosmotic coupling. Substrate level phosphorylation does not require membranes and is not the primary ATP source in these organelles.
Common Pitfalls:
Confusing substrate level phosphorylation in glycolysis or the TCA cycle with the membrane based ATP production of respiration and photosynthesis.
Final Answer:
All of the above
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