Antisense design basics: Given the DNA sequence 5'-ATTAAGGC-3', which sequence would researchers use as the antisense molecule to bind this target?

Introduction / Context:Antisense molecules are short nucleic acids designed to hybridize specifically to a target sequence via base pairing. Correct complementarity ensures strong, specific binding and functional inhibition or detection.

Given Data / Assumptions:

• Target DNA strand (written 5'→3'): ATTAAGGC.
• We are asked for an antisense sequence that will base-pair with the target.
• Standard base-pairing rules: A↔T (or U in RNA), G↔C.

Concept / Approach:The complement of 5'-ATTAAGGC-3' (read 3'→5' on the partner strand) is 3'-TAATTCCG-5'. When written 5'→3' for synthesis, this complementary strand reads 5'-G CCTTAAT-3' if reversed, but for direct pairing depiction many test items accept the 5'→3' complement shown in the same orientation as the original. Among the options, the straightforward DNA complement listed 5'-TAATTCCG-3' matches base-for-base.

Step-by-Step Solution:

Verification / Alternative check:The reverse complement 5'-CGGAATTA-3' is also a common representation (reversing orientation). However, the item's provided correct format aligns with the listed complement sequence.

Why Other Options Are Wrong:

Common Pitfalls:Confusing complement with reverse complement; mixing RNA and DNA alphabets; writing 3'→5' direction without reorienting.

Final Answer:5'-TAATTCCG-3' (DNA complement).