The usual method(s) to solve rate equation of simple enzyme kinetics is/are Mich

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Question
The usual method(s) to solve rate equation of simple enzyme kinetics is/are
Options
A. Michaelis Menten approach
B. Briggs-Haldane approach
C. Numerical solution approach
D. all of these

Correct Answer

all of these

Enzymes and Kinetics problems

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2. An enzyme has a K_m of 4.7 x 10^-5M. If the V_max of the preparation is 22m moles liter^-1 min^-1, what velocity would be observed in the presence of 2.0 x 10^-4M substrate and 5.0 x 10^-5M of a competitive inhibitor?
Options
A. 13.54? moles liter^-1min^-1
B. 6.68? moles liter^-1min^-1
C. 7.57? moles liter^-1min^-1
D. 17.8? moles liter^-1min^-1

4. Which of the following statement(s) regarding enzymes, is/are false?
Options
A. Enzymes are always proteins that function as catalysts
B. Enzymes provide activation energy for reactions
C. Enzyme activity can be regulated
D. Enzymes may be used many times for a specific reaction

5. The equation for the rate of product formation for simple enzyme reaction is given by (Where r_max, maximum reaction rate, C_s substrate concentration, C_p product concentration ES, C_ES enzyme-substrate concentration)
Options
A. r_p = r_max C_s/(K_m+C_s)
B. r_p= r_max C_ES/(K_m+ C_ES)
C. r_p = r_max C_s/(K_m+C_ES)
D. r_p = r_max C_s/(K_m+C_p)

6. When an enzyme is functioning at V_max, the rate of the reaction is limited by
Options
A. the number of collisions between enzyme and substrate
B. the number of substrate molecules in the reaction
C. the concentration of the substrate
D. the rate at which the enzyme can convert substrate to product

7. If a reaction occurs in the absence of inhibitor with rate ?₀ and in the presence of inhibitor with rate ?_i, the degree of inhibition is defined as
Options
A. (?₀ - ?_i)/?₀
B. (?₀ + ?_i)/?₀
C. (?₀?_i)/?₀
D. (?₀-?_i)/?_i

8. Which of the following step is assumed to be the slowest step in the Michaelis Menten equation?
Options
A. The substrate consuming step
B. The product releasing step
C. Formation of enzyme substrate complex
D. None of these

9. The ratio of the amount of a protein present in a sample, which is used as a measure of purification, is known as
Options
A. specific activity
B. relative activity
C. purity ratio
D. all of these

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