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  • Question
  • Saccharomyces cerevisiae produces high biomass yields at low glucose concentrations and high dissolved oxygen concentrations. Which of the following should be followed for maximizing its biomass productivity?


  • Options
  • A. Batch fermenter with a high initial glucose concentration
  • B. Continuous fermenter with a low initial glucose concentration
  • C. Fed batch fermenter
  • D. All of the above

  • Correct Answer
  • Fed batch fermenter 


  • Fermentation Reactors problems


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    • 1. The continuous cultures are not widely used in industry because

    • Options
    • A. they are not suited for the production of secondary metabolites
    • B. contamination or mutation can have a disastrous effect on the operation
    • C. the government will not approve the licensing of pharmaceuticals produced in continuous cultures
    • D. all of the above
    • Discuss
    • 2. Toxins accumulated in a reactor can lead to low biomass yields probably due to which of the following?

    • Options
    • A. NAD+ is diverted away from homeostasis to anabolism
    • B. ATP is diverted away from homeostasis to anabolism
    • C. NAD+ is diverted away from anabolism to homeostasis
    • D. ATP is diverted away from anabolism to homeostasis
    • Discuss
    • 3. Mixing profiles closest to plug flow are observed in

    • Options
    • A. continuous packed bed reactor
    • B. stirred tank reactor with biomass recycles
    • C. continuous fluidized bed bioreactor
    • D. all of these
    • Discuss
    • 4. For organisms growing in a chemostat, the specific growth rate

    • Options
    • A. cannot be determined
    • B. can be determined from the dilution rate
    • C. equals to the maximum specific growth rate of the culture
    • D. none of the above
    • Discuss
    • 5. When a culture of fermenting yeast becomes metabolically uncoupled, ethanol

    • Options
    • A. becomes a secondary metabolite
    • B. production becomes growth associated
    • C. production becomes non-growth associated
    • D. becomes a growth nutrient
    • Discuss
    • 6. A Bacillus sp. produces an antibiotic only during the stationary phase when grown in a batch culture. If grown in a 5 litre continuous culture, the steady state productivity of the antibiotic

    • Options
    • A. will increase until the culture washes out
    • B. will decrease after washout
    • C. will be zero
    • D. will be infinite
    • Discuss
    • 7. Mixing per unit volume is observed to be poorest in

    • Options
    • A. continuous packed bed reactor
    • B. continuous fluidized bed bioreactor
    • C. continuous airlift bioreactor
    • D. none of the above
    • Discuss
    • 8. A continuous reactor has a dilution rate of 0.5 h-1. Its residence time would be

    • Options
    • A. ln(2)/0.5
    • B. ln(2) x 0.5
    • C. 0.5 h
    • D. 2 h
    • Discuss
    • 9. Formation of end product by Lactococcus lactis will become non-growth associated as lactic acid accumulates because

    • Options
    • A. cells will redirect ATP to anabolism
    • B. cells will redirect NAD+ to anabolism
    • C. cells will redirect ATP to facilitate the diffusion of lactic acid and H+ out of the cells
    • D. cells will redirect ATP to the active transport of lactic acid and H+ out of the cells
    • Discuss
    • 10. Yield coefficient represents

    • Options
    • A. total biomass or product produced
    • B. conversion efficiency of a substrate into product
    • C. conversion rate of a substrate into biomass or product
    • D. production time of biomass or product
    • Discuss


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