Discussion
Home ‣ Biochemical Engineering ‣ Immobilized Enzyme Comments
- Question
Which of the following is not the correct answer?
Options- A. Immobilized enzyme may show selectively altered chemical properties
- B. Immobilized enzyme may show selectively altered physical properties
- C. Immobilized enzyme may simulate the realistic natural environment where the enzyme come from the cell
- D. None of the above
- Correct Answer
- None of the above
- 1. If Damkohler number, NDa >>1, the reaction rate is much greater than the mass transfer rate and the overall reaction is controlled by the rate of mass transfer. In this case, the enzyme reaction can be described as (where Csb and Cs are the substrate concentration in bulk of solution and at the immobilized enzyme surface respectively. ks mass transfer coefficient, and a is the surface area of an immobilized enzyme particles)
Options- A. rp = ksa(Csb-Cs)
- B. rp = ksaCsb
- C. rp = ksaCs
- D. rp = ksa(Csb+Cs) Discuss
- 2. Damkohler number(NDa) is
Options- A. the ratio of the maximum reaction rate to the maximum mass transfer rate
- B. the ratio of the minimum reaction rate to the maximum mass transfer rate
- C. the ratio of the maximum reaction rate to the minimum mass transfer rate
- D. the ratio of the minimum reaction rate to the minimum mass transfer rate Discuss
- 3. Which of the following is not a physical method of immobilization?
Options- A. Adsorption
- B. Entrapment
- C. Micro encapsulation
- D. None of these Discuss
- 4. Which medium is used for the production of Penicillin using immobilized cells
Options- A. 1% peptone medium
- B. glucose medium
- C. Yeast extract medium
- D. LB broth Discuss
- 5. Various techniques are available for determining the effective diffusivity of solute in gel. Thin disk method uses
Options- A. a diffusion cell with two compartments divided by a thin gel
- B. a diffusion cell with two compartments
- C. a diffusion cell with two compartments plus by a thin gel
- D. a diffusion cell with two compartments multiply by a thin gel Discuss
- 6. The immobilization technique involving physical method is
Options- A. covalent bond formation dependent
- B. non-covalent bond formation dependent
- C. both (a) and (b)
- D. ionic bond formation dependent Discuss
- 7. Commonly employed water insoluble supports for the covalent attachment of enzyme include
Options- A. acrylamide based polymers
- B. polypeptides
- C. dextran
- D. all of these Discuss
- 8. For a steady state condition, the change of substrate concentration (dCs/dt) is
Options- A. zero
- B. 1
- C. >1
- D. <1 Discuss
- 9. To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, ? can be defined as
Options- A. reaction rate/rate if not slowed by diffusion
- B. rate if not slowed by diffusion/ reaction rate
- C. actual reaction rate/ rate if not slowed by diffusion
- D. rate if not slowed by diffusion/ actual reaction rate Discuss
- 10. A fed-batch reactor containing 1 g.l-1 of substrate in 2 litre of medium. It is fed with 1 g.l-l of substrate at the rate of 1 litre per hour. After 10 hours, the concentration of substrate in the reactor was 0.5 g.l-1 . The mass of substrate that was used by the culture in the reactor was
Options- A. 2g
- B. 6g
- C. 8g
- D. 12g Discuss
Immobilized Enzyme problems
Search Results
Correct Answer: rp = ksaCsb
Correct Answer: the ratio of the maximum reaction rate to the maximum mass transfer rate
Correct Answer: None of these
Correct Answer: glucose medium
Correct Answer: a diffusion cell with two compartments divided by a thin gel
Correct Answer: non-covalent bond formation dependent
Correct Answer: all of these
Correct Answer: zero
Correct Answer: actual reaction rate/ rate if not slowed by diffusion
Correct Answer: 6g
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