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- Question
If Damkohler number, NDa >>1, the reaction rate is much greater than the mass transfer rate and the overall reaction is controlled by the rate of mass transfer. In this case, the enzyme reaction can be described as (where Csb and Cs are the substrate concentration in bulk of solution and at the immobilized enzyme surface respectively. ks mass transfer coefficient, and a is the surface area of an immobilized enzyme particles)
Options- A. rp = ksa(Csb-Cs)
- B. rp = ksaCsb
- C. rp = ksaCs
- D. rp = ksa(Csb+Cs)
- Correct Answer
- rp = ksaCsb
- 1. Start up expenses in a fermentation industry varies from
Options- A. 0-5% of the capital cost
- B. 10-15% of the capital cost
- C. 5-10% of the capital cost
- D. 15-20% of the capital cost Discuss
- 2. Given an enzyme with a Km = 10m M and Vmax = 100 m mol/min. If [S] = 100 m M, which of the following will be true?
Options- A. A 10 fold increase in Vmax would increase velocity 10 fold y
- B. A 10 fold decrease in Km would increase velocity
- C. Both (a) and (b)
- D. A 10 fold increase in Vmax would decrease velocity 20 fold Discuss
- 3. During fermentation, the saturation concentration of oxygen was reduced from 7 ppm. to 1 ppm. Which one of the following conclusions can be drawn?
Options- A. Microbial growth is oxygen limited since the oxygen concentration is lesser than the saturation concentration
- B. Microbial growth is not oxygen limited since the oxygen concentration is lesser than the saturation concentration
- C. Experimental error leads to the reduction
- D. No conclusion can be drawn Discuss
- 4. Power consumption by agitation is
Options- A. a function of physical properties
- B. a function of operating conditions
- C. a function of vessel and impeller geometry
- D. all of the above Discuss
- 5. The rate equation in non-competitive inhibition based on Michaelis Menten equation is given by
Options- A. rmaxS/(Km + S)(1+I/Ki)
- B. rmaxE/(Km (1+I/Ki)+S))
- C. VmaxS/(Km + S)(1+I/Ki)
- D. rmaxS/Km Discuss
- 6. The rate equation in competitive inhibition based on Michaelis Menten equation is given by
Options- A. rmaxS/(Km (1+I/Ki)+S))
- B. rmaxE/(Km (1+I/Ki)+S))
- C. rmaxI/(Km (1+I/Ki)+S))
- D. rmaxS/(Km (1+I/Ki)) Discuss
- 7. Which of the following is best described as secondary metabolite?
Options- A. Acetic acid produced from the oxidation of ethanol
- B. Ethanol from the fermentation of glucose
- C. Penicillin
- D. Citric acid from the partial oxidation of glucose Discuss
- 8. Which of the following is not correct with regards to continuous cultures?
Options- A. The major industrial scale application of continuous cultures is in waste water treatment
- B. Mutation and contamination present a major risk to the operation of continuous cultures for the production of metabolites
- C. The continuous cultures is very useful for the production of pharmaceuticals
- D. Continuous cultures have an advantage that they do not need to shut down as regularly as batch culture reactor Discuss
- 9. A fed-batch reactor containing 1 g.l-1 of substrate in 2 litre of medium. It is fed with 1 g.l-l of substrate at the rate of 1 litre per hour. After 10 hours, the concentration of substrate in the reactor was 0.5 g.l-1 . The mass of substrate that was used by the culture in the reactor was
Options- A. 2g
- B. 6g
- C. 8g
- D. 12g Discuss
- 10. In aerobic yeast fermentation for production of citric acid from alkanes using a fed-batch culture, why alkanes are slowly fed to the yeast?
Options- A. Citric acid is toxic to the cells
- B. Alkanes cause foaming
- C. Fast addition of alkanes will inhibit the cells and reduce oxygen transfer rates
- D. Fast addition of alkanes will cause the cells to grow too quickly Discuss
More questions
Correct Answer: 5-10% of the capital cost
Correct Answer: A 10 fold increase in Vmax would increase velocity 10 fold y
Correct Answer: No conclusion can be drawn
Correct Answer: all of the above
Correct Answer: rmaxS/(Km + S)(1+I/Ki)
Correct Answer: rmaxS/(Km (1+I/Ki)+S))
Correct Answer: Penicillin
Correct Answer: The continuous cultures is very useful for the production of pharmaceuticals
Correct Answer: 6g
Correct Answer: Fast addition of alkanes will inhibit the cells and reduce oxygen transfer rates
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