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Home ‣ Biochemical Engineering ‣ Downstream Processing Comments
  • Question

  • The correlation often employed to correlate adsorption data for protein is (where Ymax is the maximum amount of solute adsorbed per mass of adsorbent, X is the mass fraction of solute in the diluent phase in solute-free basis, KL is a constant and Y is the equilibrium value of the mass of solute adsorbed per mass of adsorbent)


  • Options
  • A. Y = YmaxX/(KL+X)
  • B. Y = Ymax/(KL+X)
  • C. Y = YmaxX/KL
  • D. Y = Ymax- X/ (KL+X)

  • Correct Answer
  • Y = YmaxX/(KL+X) 

    • Downstream Processing problems

    Search Results

    • 1. Which of the following is considered as a pretreatment to the biological feeds?

    • Options
    • A. Heating to denature the proteins
    • B. Addition of filter aids to increase the porosity
    • C. Addition of electrolytes
    • D. All of the above
      • Discuss
    • 2. Micro filtration refers to the separation of suspended material such as bacteria by using a membrane with spore sizes of

    • Options
    • A. 0.02 to 10?m
    • B. 1-10A°
    • C. 20-30?m
    • D. 10-200A°
      • Discuss
    • 3. The conventional filtration involves the separation of large particles generally

    • Options
    • A. dp>5?m
    • B. dp>10?m
    • C. dp>15?m
    • D. dp>20?m
      • Discuss
    • 4. If the solute passes through membrane freely, the the rejection coefficient (s) is

    • Options
    • A. 1
    • B. 0
    • C. >1
    • D. <1
      • Discuss
    • 5. Conventional adsorption is a

    • Options
    • A. reversible process
    • B. irreversible process
    • C. either reversible or irreversible
    • D. none of these
      • Discuss
    • 6. Which is not the method for producing immobilized enzymes with multifunctional reagents?

    • Options
    • A. Enzymes are adsorbed on the surface active support followed by intermolecular cross linking
    • B. Functional groups are introduced on the support to react co-valently with enzymes
    • C. Enzymes are cross linked intermolecularly
    • D. None of the above
      • Discuss
    • 7. The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by

    • Options
    • A. copolymerization or microencapsulation
    • B. crosslinking using multifunctional reagents
    • C. adsorption
    • D. all of the above
      • Discuss
    • 8. The support material for immobilization of cells of Bacillus subtilis is

    • Options
    • A. ion exchange resins
    • B. gelatin
    • C. Anthracite
    • D. agarose and carbodiimide
      • Discuss
    • 9. Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have

    • Options
    • A. low molecular weight
    • B. high molecular weight
    • C. high equivalent weight
    • D. low reactivity
      • Discuss
    • 10. The immobilized technique involving chemical method is

    • Options
    • A. covalent bond formation dependent
    • B. non-covalent bond formation dependent
    • C. both (a) and (b)
    • D. ionic bond formation dependent
      • Discuss

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