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- Question
Which of the followings are the metabolic products of glucose and glutamine?
Options- A. CO2 and NH3
- B. CO2 and lactate
- C. Lactate and ammonium
- D. Lactate only
- Correct Answer
- Lactate and ammonium
- 1. In animal cell culture, particularly mammalian cell culture, transformation means
Options- A. uptake of new genetic material
- B. phenotypic modifications of cells in culture
- C. both (a) and (b)
- D. release of genetic information Discuss
- 2. For culturing, plasma from the adult chicken is preferred to mammalian plasma because
Options- A. it forms a clear and solid coagulum even after dilution
- B. it is too opaque
- C. it doesn't produce solid clots
- D. it forms a semi solid coagulum Discuss
- 3. Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?
Options- A. Down's syndrome
- B. Turner's syndrome
- C. Klinefelter's syndrome
- D. all of these Discuss
- 4. Cells which have undergone transformation frequently become
Options- A. anchorage independent
- B. anchorage dependent
- C. stable
- D. unstable Discuss
- 5. During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?
Options- A. Ethyl alcohol is being produced in excess
- B. The cells have too much oxygen
- C. Glycolysis is being inhibited
- D. The cells do not have enough oxygen Discuss
- 6. An established cell line can be called where it has been subcultured at least
Options- A. 70 times at an interval of 3 days between subcultures
- B. 40 times at an interval of 3 days between subcultures
- C. 70 times at an interval of 1 day between subcultures
- D. 50 times at an interval of 3 days between subcultures Discuss
- 7. Which of the following is not the explantation technique?
Options- A. Slide culture
- B. Carrel flask culture
- C. Roller test tube culture
- D. Adherent primary culture Discuss
- 8. Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is
Options- A. 150-300
- B. 280-360
- C. 300-325
- D. 360-400 Discuss
- 9. Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)
Options- A. 0.05-5 nmol of O2/cell/h
- B. 5 - 10 nmol of O2 /cell/h
- C. 0.05-5 pmol of O2/cell/h
- D. 5 - 10 pmol of O2/cell/h Discuss
- 10. Range of optimum glucose concentration present in the culture media is
Options- A. 5.5 - 55 mmol/litre
- B. 55 - 75 mmol/litre
- C. 75-105 mmol/litre
- D. 105-150 mmol/litre Discuss
Animal Cell Culture and Regulation problems
Search Results
Correct Answer: phenotypic modifications of cells in culture
Correct Answer: it forms a clear and solid coagulum even after dilution
Correct Answer: all of these
Correct Answer: anchorage independent
Correct Answer: The cells do not have enough oxygen
Correct Answer: 70 times at an interval of 3 days between subcultures
Correct Answer: Adherent primary culture
Correct Answer: 280-360
Correct Answer: 0.05-5 pmol of O2/cell/h
Correct Answer: 5.5 - 55 mmol/litre
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