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Home Biotechnology Animal Cell Culture and Regulation Comments

  • Question
  • In animal cell culture, particularly mammalian cell culture, transformation means


  • Options
  • A. uptake of new genetic material
  • B. phenotypic modifications of cells in culture
  • C. both (a) and (b)
  • D. release of genetic information

  • Correct Answer
  • phenotypic modifications of cells in culture 


  • Animal Cell Culture and Regulation problems


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    • 1. For culturing, plasma from the adult chicken is preferred to mammalian plasma because

    • Options
    • A. it forms a clear and solid coagulum even after dilution
    • B. it is too opaque
    • C. it doesn't produce solid clots
    • D. it forms a semi solid coagulum
    • Discuss
    • 2. Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

    • Options
    • A. Down's syndrome
    • B. Turner's syndrome
    • C. Klinefelter's syndrome
    • D. all of these
    • Discuss
    • 3. Cells which have undergone transformation frequently become

    • Options
    • A. anchorage independent
    • B. anchorage dependent
    • C. stable
    • D. unstable
    • Discuss
    • 4. During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?

    • Options
    • A. Ethyl alcohol is being produced in excess
    • B. The cells have too much oxygen
    • C. Glycolysis is being inhibited
    • D. The cells do not have enough oxygen
    • Discuss
    • 5. When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

    • Options
    • A. incomplete glutamine oxidation
    • B. increase in specific lactate production from glucose
    • C. both (a) and (b)
    • D. accumulation of ammonia
    • Discuss
    • 6. Which of the followings are the metabolic products of glucose and glutamine?

    • Options
    • A. CO2 and NH3
    • B. CO2 and lactate
    • C. Lactate and ammonium
    • D. Lactate only
    • Discuss
    • 7. An established cell line can be called where it has been subcultured at least

    • Options
    • A. 70 times at an interval of 3 days between subcultures
    • B. 40 times at an interval of 3 days between subcultures
    • C. 70 times at an interval of 1 day between subcultures
    • D. 50 times at an interval of 3 days between subcultures
    • Discuss
    • 8. Which of the following is not the explantation technique?

    • Options
    • A. Slide culture
    • B. Carrel flask culture
    • C. Roller test tube culture
    • D. Adherent primary culture
    • Discuss
    • 9. Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

    • Options
    • A. 150-300
    • B. 280-360
    • C. 300-325
    • D. 360-400
    • Discuss
    • 10. Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

    • Options
    • A. 0.05-5 nmol of O2/cell/h
    • B. 5 - 10 nmol of O2 /cell/h
    • C. 0.05-5 pmol of O2/cell/h
    • D. 5 - 10 pmol of O2/cell/h
    • Discuss


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