Discussion
Home ‣ Biotechnology ‣ Animal Cell Culture and Regulation Comments
- Question
In animal cell culture, particularly mammalian cell culture, transformation means
Options- A. uptake of new genetic material
- B. phenotypic modifications of cells in culture
- C. both (a) and (b)
- D. release of genetic information
- Correct Answer
- phenotypic modifications of cells in culture
- 1. For culturing, plasma from the adult chicken is preferred to mammalian plasma because
Options- A. it forms a clear and solid coagulum even after dilution
- B. it is too opaque
- C. it doesn't produce solid clots
- D. it forms a semi solid coagulum Discuss
- 2. Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?
Options- A. Down's syndrome
- B. Turner's syndrome
- C. Klinefelter's syndrome
- D. all of these Discuss
- 3. Cells which have undergone transformation frequently become
Options- A. anchorage independent
- B. anchorage dependent
- C. stable
- D. unstable Discuss
- 4. During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?
Options- A. Ethyl alcohol is being produced in excess
- B. The cells have too much oxygen
- C. Glycolysis is being inhibited
- D. The cells do not have enough oxygen Discuss
- 5. When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of
Options- A. incomplete glutamine oxidation
- B. increase in specific lactate production from glucose
- C. both (a) and (b)
- D. accumulation of ammonia Discuss
- 6. Which of the followings are the metabolic products of glucose and glutamine?
Options- A. CO2 and NH3
- B. CO2 and lactate
- C. Lactate and ammonium
- D. Lactate only Discuss
- 7. An established cell line can be called where it has been subcultured at least
Options- A. 70 times at an interval of 3 days between subcultures
- B. 40 times at an interval of 3 days between subcultures
- C. 70 times at an interval of 1 day between subcultures
- D. 50 times at an interval of 3 days between subcultures Discuss
- 8. Which of the following is not the explantation technique?
Options- A. Slide culture
- B. Carrel flask culture
- C. Roller test tube culture
- D. Adherent primary culture Discuss
- 9. Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is
Options- A. 150-300
- B. 280-360
- C. 300-325
- D. 360-400 Discuss
- 10. Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)
Options- A. 0.05-5 nmol of O2/cell/h
- B. 5 - 10 nmol of O2 /cell/h
- C. 0.05-5 pmol of O2/cell/h
- D. 5 - 10 pmol of O2/cell/h Discuss
Animal Cell Culture and Regulation problems
Search Results
Correct Answer: it forms a clear and solid coagulum even after dilution
Correct Answer: all of these
Correct Answer: anchorage independent
Correct Answer: The cells do not have enough oxygen
Correct Answer: both (a) and (b)
Correct Answer: Lactate and ammonium
Correct Answer: 70 times at an interval of 3 days between subcultures
Correct Answer: Adherent primary culture
Correct Answer: 280-360
Correct Answer: 0.05-5 pmol of O2/cell/h
Comments
There are no comments.More in Biotechnology:
Programming
Copyright ©CuriousTab. All rights reserved.