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- Question
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?
Options- A. Ethyl alcohol is being produced in excess
- B. The cells have too much oxygen
- C. Glycolysis is being inhibited
- D. The cells do not have enough oxygen
- Correct Answer
- The cells do not have enough oxygen
- 1. When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of
Options- A. incomplete glutamine oxidation
- B. increase in specific lactate production from glucose
- C. both (a) and (b)
- D. accumulation of ammonia Discuss
- 2. The human fibroplast is a classical example of
Options- A. stable primary cell lines
- B. established cell lines
- C. cell transformations
- D. none of these Discuss
- 3. Which of the following is not a source of energy in active muscle cells?
Options- A. Creatine phosphate
- B. ATP
- C. Lactic acid
- D. Glucose Discuss
- 4. Range of optimum glutamine concentration present in the culture media is
Options- A. 1-2 mmol/litre
- B. 2-7 mmol/litre
- C. 7-15 mmol/litre
- D. 15 - 20 mmol/litre Discuss
- 5. Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called
Options- A. established cell lines
- B. primary cell lines
- C. secondary cell lines
- D. propagated cell lines Discuss
- 6. Cells which have undergone transformation frequently become
Options- A. anchorage independent
- B. anchorage dependent
- C. stable
- D. unstable Discuss
- 7. Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?
Options- A. Down's syndrome
- B. Turner's syndrome
- C. Klinefelter's syndrome
- D. all of these Discuss
- 8. For culturing, plasma from the adult chicken is preferred to mammalian plasma because
Options- A. it forms a clear and solid coagulum even after dilution
- B. it is too opaque
- C. it doesn't produce solid clots
- D. it forms a semi solid coagulum Discuss
- 9. In animal cell culture, particularly mammalian cell culture, transformation means
Options- A. uptake of new genetic material
- B. phenotypic modifications of cells in culture
- C. both (a) and (b)
- D. release of genetic information Discuss
- 10. Which of the followings are the metabolic products of glucose and glutamine?
Options- A. CO2 and NH3
- B. CO2 and lactate
- C. Lactate and ammonium
- D. Lactate only Discuss
Animal Cell Culture and Regulation problems
Search Results
Correct Answer: both (a) and (b)
Correct Answer: stable primary cell lines
Correct Answer: Lactic acid
Correct Answer: 2-7 mmol/litre
Correct Answer: established cell lines
Correct Answer: anchorage independent
Correct Answer: all of these
Correct Answer: it forms a clear and solid coagulum even after dilution
Correct Answer: phenotypic modifications of cells in culture
Correct Answer: Lactate and ammonium
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