logo

CuriousTab

CuriousTab

Discussion


Home Biotechnology Animal Cell Culture and Regulation Comments

  • Question
  • During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?


  • Options
  • A. Ethyl alcohol is being produced in excess
  • B. The cells have too much oxygen
  • C. Glycolysis is being inhibited
  • D. The cells do not have enough oxygen

  • Correct Answer
  • The cells do not have enough oxygen 


  • Animal Cell Culture and Regulation problems


    Search Results


    • 1. When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

    • Options
    • A. incomplete glutamine oxidation
    • B. increase in specific lactate production from glucose
    • C. both (a) and (b)
    • D. accumulation of ammonia
    • Discuss
    • 2. The human fibroplast is a classical example of

    • Options
    • A. stable primary cell lines
    • B. established cell lines
    • C. cell transformations
    • D. none of these
    • Discuss
    • 3. Which of the following is not a source of energy in active muscle cells?

    • Options
    • A. Creatine phosphate
    • B. ATP
    • C. Lactic acid
    • D. Glucose
    • Discuss
    • 4. Range of optimum glutamine concentration present in the culture media is

    • Options
    • A. 1-2 mmol/litre
    • B. 2-7 mmol/litre
    • C. 7-15 mmol/litre
    • D. 15 - 20 mmol/litre
    • Discuss
    • 5. Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

    • Options
    • A. established cell lines
    • B. primary cell lines
    • C. secondary cell lines
    • D. propagated cell lines
    • Discuss
    • 6. Cells which have undergone transformation frequently become

    • Options
    • A. anchorage independent
    • B. anchorage dependent
    • C. stable
    • D. unstable
    • Discuss
    • 7. Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

    • Options
    • A. Down's syndrome
    • B. Turner's syndrome
    • C. Klinefelter's syndrome
    • D. all of these
    • Discuss
    • 8. For culturing, plasma from the adult chicken is preferred to mammalian plasma because

    • Options
    • A. it forms a clear and solid coagulum even after dilution
    • B. it is too opaque
    • C. it doesn't produce solid clots
    • D. it forms a semi solid coagulum
    • Discuss
    • 9. In animal cell culture, particularly mammalian cell culture, transformation means

    • Options
    • A. uptake of new genetic material
    • B. phenotypic modifications of cells in culture
    • C. both (a) and (b)
    • D. release of genetic information
    • Discuss
    • 10. Which of the followings are the metabolic products of glucose and glutamine?

    • Options
    • A. CO2 and NH3
    • B. CO2 and lactate
    • C. Lactate and ammonium
    • D. Lactate only
    • Discuss


    Comments

    There are no comments.

Enter a new Comment