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Home Biotechnology Animal Cell Culture and Regulation Comments

  • Question
  • The human fibroplast is a classical example of


  • Options
  • A. stable primary cell lines
  • B. established cell lines
  • C. cell transformations
  • D. none of these

  • Correct Answer
  • stable primary cell lines 


  • Animal Cell Culture and Regulation problems


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    • 1. Which of the following is not a source of energy in active muscle cells?

    • Options
    • A. Creatine phosphate
    • B. ATP
    • C. Lactic acid
    • D. Glucose
    • Discuss
    • 2. Range of optimum glutamine concentration present in the culture media is

    • Options
    • A. 1-2 mmol/litre
    • B. 2-7 mmol/litre
    • C. 7-15 mmol/litre
    • D. 15 - 20 mmol/litre
    • Discuss
    • 3. Sometimes cell lines can be cultured for such a long time that they apparently develop the potential to be subcultured indefinitely in vitro. Such cells lines are called

    • Options
    • A. established cell lines
    • B. primary cell lines
    • C. secondary cell lines
    • D. propagated cell lines
    • Discuss
    • 4. What are the main constituents of culture for animal cell growth?

    • Options
    • A. Glucose and Glutamine
    • B. Growth factors
    • C. Cytokines
    • D. All of these
    • Discuss
    • 5. The major problem associated with the isolation of free cells and cell aggregates from organs is that of

    • Options
    • A. releasing the cells from their supporting matrix
    • B. inhibiting the cells from their supporting matrix
    • C. disintegrating the cells from their supporting matrix
    • D. none of the above
    • Discuss
    • 6. When dissolved oxygen is lower than the critical concentration, viable cell concentration declines because of

    • Options
    • A. incomplete glutamine oxidation
    • B. increase in specific lactate production from glucose
    • C. both (a) and (b)
    • D. accumulation of ammonia
    • Discuss
    • 7. During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?

    • Options
    • A. Ethyl alcohol is being produced in excess
    • B. The cells have too much oxygen
    • C. Glycolysis is being inhibited
    • D. The cells do not have enough oxygen
    • Discuss
    • 8. Cells which have undergone transformation frequently become

    • Options
    • A. anchorage independent
    • B. anchorage dependent
    • C. stable
    • D. unstable
    • Discuss
    • 9. Which of the following abnormality, resulted from the inheritance of an unbalanced complement of chromosomes can be diagnosed through karotyping?

    • Options
    • A. Down's syndrome
    • B. Turner's syndrome
    • C. Klinefelter's syndrome
    • D. all of these
    • Discuss
    • 10. For culturing, plasma from the adult chicken is preferred to mammalian plasma because

    • Options
    • A. it forms a clear and solid coagulum even after dilution
    • B. it is too opaque
    • C. it doesn't produce solid clots
    • D. it forms a semi solid coagulum
    • Discuss


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