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Home Biotechnology Media Required for Plant Cell Culture Comments

  • Question
  • To maintain the pH of the culture


  • Options
  • A. organic acid such as citric, fumaric, malic and succinic acid is used
  • B. synthetic buffers such as Tris, MES or HEPS are used
  • C. both (a) and (b)
  • D. ammonium salts are used

  • Correct Answer
  • both (a) and (b) 


  • Media Required for Plant Cell Culture problems


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    • 1. Which of the following growth regulator is used to stimulate embryo or shoot development?

    • Options
    • A. Auxins
    • B. Cytokinins
    • C. Gibberellins
    • D. Brassinosteroids
    • Discuss
    • 2. Which of the following is not true about nurse or conditioned medium?

    • Options
    • A. It is liquid removed from the suspension of fast growing cells
    • B. It contains uncharacterized growth factor released by growing cells
    • C. It is used in the culture of regenerating protoplast
    • D. it is removed aseptically from the culture and is autoclaved before use
    • Discuss
    • 3. Concentration of sucrose generally used in plant cell culture media is

    • Options
    • A. 10-15 g/l
    • B. 20-30 g/l
    • C. 40-50 g/l
    • D. 60-70 g/l
    • Discuss
    • 4. Which of the following is an ethylene biosynthesis inhibitor?

    • Options
    • A. Citric acid
    • B. Succinic acid
    • C. Activated charcoal
    • D. Silver thiosulphate
    • Discuss
    • 5. Which is the most common carbon source used in the plant cell culture media?

    • Options
    • A. Sucrose
    • B. Glucose
    • C. Fructose
    • D. Maltose
    • Discuss
    • 6. Starch content of potatoes can be increased by using a bacterial gene, known as

    • Options
    • A. sucrose phosphate synthase gene
    • B. ADP glucose pyrophosphorylase gene
    • C. polygalactouranase gene
    • D. none of the above
    • Discuss
    • 7. What are the various disadvantages of cross protection?

    • Options
    • A. Possibility of mutations in inducing mild virus strain
    • B. Possibility of synergism between inducing virus and other unrelated virus
    • C. Possibility of unnecessary spread of mild virus
    • D. All of the above
    • Discuss
    • 8. Insect resistance in the transgenic plant has been achieved by

    • Options
    • A. transferring genes for Bt toxins
    • B. transferring genes for protease inhibitors
    • C. transferring genes for other insecticidal secondary metabolities
    • D. all of the above
    • Discuss
    • 9. Antisense technology

    • Options
    • A. selectively blocks expression of a gene
    • B. combines genetic material from different species
    • C. combines organelles and cells
    • D. alters or transfers cells
    • Discuss
    • 10. Resistance to glyphosphate in transgenic petunia plants has been developed by the transfer of

    • Options
    • A. gene for EPSPS(5-enol-pyruvyl shikimat 3 phosphate synthase)
    • B. gene for ALS (acetolactate synthase)
    • C. gene for GS (glutamine synthase)
    • D. any of the above
    • Discuss


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