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Home Biotechnology Vectors Uses for Animal Cell Culture Comments

  • Question
  • In case of Baculoviruses, the foreign gene


  • Options
  • A. is inserted downstream of polyhedrin promoter
  • B. is inserted downstream of viral origin of replication
  • C. is inserted between the polyhedrin promoter and downstream sequences
  • D. is inserted randomly in viral genome

  • Correct Answer
  • is inserted between the polyhedrin promoter and downstream sequences 


  • Vectors Uses for Animal Cell Culture problems


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    • 1. Which of the following promoters is repressed in presence of glucose?

    • Options
    • A. Galactokinase (GAL 1)
    • B. Acid phosphatase (PHOS)
    • C. Both (a) and (b)
    • D. Phosphoglycerate kinase (PGK)
    • Discuss
    • 2. Maximum amount of foreign DNA introduced into SV 40 viruses is

    • Options
    • A. 10 kb
    • B. 5 kb
    • C. 2.5 kb
    • D. 1 kb
    • Discuss
    • 3. Autonomously replicating sequence is not present in

    • Options
    • A. YIp
    • B. YRp
    • C. YCp
    • D. YAC
    • Discuss
    • 4. Which of the following can be used for reducing the size of gene inserts containing introns?

    • Options
    • A. Vaccinia virus
    • B. Baculo virus
    • C. Retro virus
    • D. none of these
    • Discuss
    • 5. Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

    • Options
    • A. Contains centromeric sequences
    • B. Segregates during meiosis and mitosis
    • C. Functions as true chromosome
    • D. All of the above
    • Discuss
    • 6. Bovine papilloma virus has a circular double stranded DNA genome of about

    • Options
    • A. 2.5 kb
    • B. 5.2 kb
    • C. 7.9 kb
    • D. 9.5 kb
    • Discuss
    • 7. Which of the following viruses are used as vectors for transferring genes to animal cells?

    • Options
    • A. SV 40 virus
    • B. Bovine papilloma virus (BVP)
    • C. Retroviruses
    • D. All of these
    • Discuss
    • 8. Moloney-Mu-leukemia virus is an example of

    • Options
    • A. Vaccinia virus
    • B. Baculo virus
    • C. Retro virus
    • D. none of these
    • Discuss
    • 9. Cloning genes in the yeast offer which of following advantage(s)?

    • Options
    • A. Over production of proteins of commercial value
    • B. Ability to glycosylate proteins during secretion
    • C. Ability to clone large piece of DNA
    • D. All of the above
    • Discuss
    • 10. α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

    • Options
    • A. His 3
    • B. Lys 2
    • C. Ura 3
    • D. Trp 1
    • Discuss


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