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Home Biochemistry UV Luminance Spectroscopy Comments

  • Question
  • Why must the radiation source for fluorescence spectrometry be more powerful than for absorption spectroscopy?


  • Options
  • A. Because the magnitude of the output signal is proportional to the power of the incident radiation
  • B. Because the sample won't fluoresce if the incident radiation is of low power
  • C. To allow for scattering by the sample
  • D. None of the above

  • Correct Answer
  • Because the magnitude of the output signal is proportional to the power of the incident radiation 


  • UV Luminance Spectroscopy problems


    Search Results


    • 1. What is the relationship between wavelength and wave number?

    • Options
    • A. Wavenumber = 1 / wavelength in centimeters
    • B. Wavenumber - wavelength in nanometers = 1
    • C. Wavelength in nanometers x wavenumber = 1
    • D. None of the above
    • Discuss
    • 2. Where does a carbonyl (C=0) stretch appear in an IR spectrum?

    • Options
    • A. 1740-1720
    • B. 1870-1650
    • C. 3640-3250
    • D. 160-110
    • Discuss
    • 3. Which of the following will oscillate the fastest?

    • Options
    • A. A large mass on a weak spring
    • B. A large mass on a stiff spring
    • C. A small mass on a stiff spring
    • D. A small mass on a weak spring
    • Discuss
    • 4. Which of the following is not an IR vibrational mode?

    • Options
    • A. Stretching
    • B. Scissoring
    • C. Rocking
    • D. Rolling
    • Discuss
    • 5. Why must the voltage supplied to a tungsten lamp be very stable?

    • Options
    • A. Because if it wasn't, the lamp would bum out
    • B. Because amount of energy the lamp emits is proportional to the fourth power of the operating voltage
    • C. Because the lamp will only function at a specific voltage
    • D. All of the above
    • Discuss
    • 6. Why phosphorescence measurements are usually made at a low temperature?

    • Options
    • A. To prevent thermal degradation of the phosphorescent species
    • B. To promote phosphorescence by slowing the rate of radiationless transfer processes
    • C. To increase the efficiency of the detector
    • D. To decease the efficiency of detector
    • Discuss
    • 7. In the intersystem crossing

    • Options
    • A. the spin of an excited electron reverses, changing the state of the molecule (from singlet state to triplet state or vice versa)
    • B. a molecule converts its excess energy to light, and emits a photon
    • C. a molecule converts excess electronic energy to vibrational energy
    • D. all of the above
    • Discuss
    • 8. For a molecule to absorb IR, why must the molecule's vibrations cause fluctuations in the dipole moment of the molecule?

    • Options
    • A. Because a change in dipole moment lowers the energy required for electronic transitions
    • B. Because for absorption to occur, the radiation must interact with the electric field caused by changing dipole moment
    • C. Because fluctuations in the dipole moment allow the molecule to deform by bending and stretching
    • D. All of the above
    • Discuss
    • 9. Why do fluorescence spectrometers often use double-beam optics?

    • Options
    • A. So a reference solution can be used
    • B. To compensate for beam attenuation by the monochromator
    • C. To compensate for power fluctuations in the radiation source
    • D. All of the above
    • Discuss
    • 10. Fluorescence occurs when

    • Options
    • A. a molecule returns to the electronic ground state from an excited triplet state by losing it's excess energy as a photon
    • B. a molecule returns to the electronic ground state from an excited singlet state by losing it's excess energy as a photon
    • C. a molecule lowers its vibrational energy by losing it's excess energy as a photon
    • D. none of the above
    • Discuss


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