Why must the radiation source for fluorescence spectrometry be more powerful than for absorption spectroscopy?
Options
A. Because the magnitude of the output signal is proportional to the power of the incident radiation
B. Because the sample won't fluoresce if the incident radiation is of low power
C. To allow for scattering by the sample
D. None of the above
Correct Answer
Because the magnitude of the output signal is proportional to the power of the incident radiation
More questions
1. In the study of one experiment it was found that the value of Tm for DNA is = 40° C. If the cell has 20% GC at the above Tm, then what will be value of 'Tm' if the GC% increases to 60%?