Difficulty: Easy
Correct Answer: Protoplast
Explanation:
Introduction / Context:
Removing the cell wall allows researchers to manipulate cells directly, fuse them, or introduce DNA and organelles more readily. The standard term varies by organism: in plants, the wall-less cell is called a protoplast; in some bacteria, partially wall-deficient forms are called spheroplasts.
Given Data / Assumptions:
Concept / Approach:
A protoplast encompasses the living content of the cell bounded by the plasma membrane after the rigid wall is removed. This enables protoplast fusion, somatic hybridization, and efficient DNA uptake techniques in plant biotechnology.
Step-by-Step Solution:
Verification / Alternative check:
Protocols in plant tissue culture routinely generate protoplasts for regeneration into whole plants under proper conditions.
Why Other Options Are Wrong:
Common Pitfalls:
Using “spheroplast” for plant cells; the accepted plant term is “protoplast.”
Final Answer:
Protoplast
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