In industrial microbiology, Candida utilis (also known as Torula yeast) is used to ferment spent sulphite (sulfite) waste liquor from the pulp and paper industry. This feedstock contains a mixture of wood-derived sugars. Which sugars can C. utilis efficiently ferment in this process?

Difficulty: Easy

Correct Answer: Both hexoses and pentoses

Explanation:


Introduction / Context:
Spent sulphite liquor is a by-product of wood pulping that contains a complex mixture of soluble sugars released from hemicellulose and cellulose. Selecting a microorganism that can utilize multiple sugar types is essential for efficient fermentation and waste valorization. Candida utilis is widely applied because it can metabolize a broad spectrum of sugars, enabling high conversion yields and single-cell protein production.


Given Data / Assumptions:

  • Feedstock: spent sulphite liquor rich in glucose, mannose, xylose, and other wood sugars.
  • Organism: Candida utilis (Torula yeast) adapted to lignocellulosic hydrolysates.
  • Goal: maximize sugar utilization and fermentation efficiency.


Concept / Approach:
The key requirement is pentose- and hexose-fermenting capacity. Many conventional yeasts (e.g., Saccharomyces cerevisiae) readily ferment hexoses but poorly ferment pentoses without engineering. C. utilis, however, can assimilate both sugar classes, making it suitable for mixed-sugar hydrolysates typical of lignocellulosic streams.


Step-by-Step Solution:

Identify sugar composition of spent sulphite liquor: contains hexoses (glucose, mannose) and pentoses (xylose, arabinose).Evaluate organism capabilities: C. utilis can utilize both sugar types for growth and product formation.Infer best match: the organism’s broad substrate range aligns with mixed-sugar feedstocks.Select the option indicating utilization of both hexoses and pentoses.


Verification / Alternative check:
Industrial practice and literature on single-cell protein production from pulp mill liquors consistently cite C. utilis because of its pentose assimilation, confirming the choice.


Why Other Options Are Wrong:

  • Hexoses only: ignores pentose-rich hemicellulose sugars.
  • Pentoses only: C. utilis also ferments hexoses efficiently.
  • None/Disaccharides only: contradicts established metabolic breadth.


Common Pitfalls:
Assuming all yeasts behave like S. cerevisiae; failing to consider the pentose fraction of lignocellulosic hydrolysates.


Final Answer:
Both hexoses and pentoses

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