Downstream Processing Questions

• In antibiotic manufacturing processes, the fermentation time ranges from
• Chromatography is based on the
• A common filter medium is the cloth filter generally made of
• Maintenance cost varies from
• The stage wise operation of adsorption is called
• The purity of a solute collected between two times t1 and t2 during chromatographic separation can be calculated as
• Start up expenses in a fermentation industry varies from
• Selectivity can be expressed as
• Concentration polarization can be reduced further by
• The solvent flux across the membrane can be expressed as (where A is the area, Lp is the membrane permeability, σ is the reflection point, Δp is applied pressure and Δπ is the osmotic pressure)
• A typical tubular centrifuge has a bowl of 2 to 5 inch in diameter and 9 to 30 inch in height with a maximum rotating speed of
• The disk centrifuge is the type of centrifuge used most often for bio separations due to its
• Which of the following is not the physical method for the cells rupturing?
• The optimum wet solid content for the cell suspension for a bead mill is typically somewhere between
• The effectiveness of a solvent can be measured by the
• A system which require less solvent and produces a more concentrated extract phase, is desired with a
• If the membrane rejects all solute, the rejection coefficient (s) is
• Assuming the laminar flow across the filter, the rate of filtration (dVf/dt) can be expressed as a function of pressure drop Dp by the modified D'Arcy's equation as (where A is the area of filtering surface, K is the D'Arcy's filter cake permeability and L is the thickness of the filter cake)
• Filtration leaf test results indicate that the filtration rate of a protein product is 100 dry lbs/(ft2 hr). What size production filter would be required to obtain 100 dry lbs of filter cake/hr?
• Which of the operation does not come under upstream processing?