Difficulty: Easy
Correct Answer: Bacteria take up free DNA directly from their environment.
Explanation:
Introduction / Context:
Bacteria can exchange genetic material in several ways, which is important for evolution, adaptation, and the spread of traits such as antibiotic resistance. The three classic mechanisms are transformation, transduction, and conjugation. This question requires you to recognize which description best matches transformation specifically, a fundamental concept in microbiology and genetics.
Given Data / Assumptions:
Concept / Approach:
Transformation is the process by which a bacterial cell takes up free DNA from its surroundings, usually from dead or lysed cells. The DNA may then recombine with the recipient chromosome or exist as a plasmid. Transduction, in contrast, involves bacteriophages that carry DNA from one bacterium to another. Conjugation involves direct cell to cell contact and a pilus. Dormancy is a survival strategy such as spore formation and is not a mechanism of gene transfer. Therefore, the correct answer must emphasize uptake of free environmental DNA by bacteria.
Step-by-Step Solution:
Step 1: Recall that transformation does not require bacteriophages or direct cell contact.
Step 2: Recognize that in transformation, competent bacteria bind and internalize naked DNA that is present in their environment.
Step 3: Compare each option and find the one that clearly describes uptake of environmental DNA.
Step 4: Select the option about bacteria taking DNA from their environment as the correct description of transformation.
Verification / Alternative check:
Historical experiments such as Griffiths work with Streptococcus pneumoniae demonstrated transformation when non virulent bacteria acquired virulence from heat killed virulent cells through DNA uptake. This classic example matches the description of bacteria taking DNA from their environment and helps confirm the correct choice.
Why Other Options Are Wrong:
Injection of DNA into another cell: This resembles aspects of conjugation or bacteriophage infection but does not define transformation.
Dormant for many weeks: This describes survival strategies like spore formation or stationary phase, not gene transfer.
Transfer with a bacteriophage: This is transduction, in which viruses shuttle DNA between bacterial cells.
Common Pitfalls:
Students often confuse transformation with transduction or conjugation because all involve DNA movement. To avoid confusion, remember key words: naked DNA uptake for transformation, virus mediated transfer for transduction, and pilus mediated cell contact for conjugation. Keeping these definitions clear will help answer many microbiology questions accurately.
Final Answer:
Transformation in bacteria is best described as the process in which bacteria take up free DNA directly from their environment.
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