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Home Biochemistry FT IR Spectroscopy Comments

  • Question
  • What is meant by rotating frame of reference?


  • Options
  • A. That the sample is spun rapidly in the applied field
  • B. If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space
  • C. That the detector rotates around the sample
  • D. None of the above

  • Correct Answer
  • If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space 


  • FT IR Spectroscopy problems


    Search Results


    • 1. In immunofluroscence microscopy, fluroscent compounds are attached to

    • Options
    • A. an antibody specific for the subcellular structure
    • B. an antigen specific for the subcellular structure
    • C. bound antibody specific for the subcellular structure
    • D. none of these
    • Discuss
    • 2. In FT-NMR, how are nuclei excited?

    • Options
    • A. By radio-frequency radiation whose frequency is swept across a predetermined range
    • B. By an intense pulse of radiation which contains a wide range of frequencies
    • C. By an intense pressure
    • D. None of the above
    • Discuss
    • 3. How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field?

    • Options
    • A. 2
    • B. 4
    • C. 3
    • D. 6
    • Discuss
    • 4. In scanning electron microscopy

    • Options
    • A. a specimen is fixed and then coated with thin layer of a heavy metal
    • B. a specimen is fixed and then coated with transition metal
    • C. a specimen is not fixed and then coated with thin layer of a heavy metal
    • D. none of the above
    • Discuss
    • 5. Negative staining is a technique used in

    • Options
    • A. electron microscopy
    • B. gel electrophoresis
    • C. immunocytochemistry
    • D. light microscopy
    • Discuss
    • 6. The sequence of amino acids in proteins can be determined by means of

    • Options
    • A. identification of the -NH2 terminal amino acids
    • B. identification of the -COOH terminal amino acids
    • C. partial cleavage of the original polypeptide into smaller polypeptides
    • D. all of the above
    • Discuss
    • 7. The frequency of precession, the transition frequency and the Larmor frequency are

    • Options
    • A. different terms for the same frequency
    • B. same terms for the same frequency
    • C. different terms for the different frequency
    • D. same terms for the different frequency
    • Discuss
    • 8. What does the Michelson interferometer do?

    • Options
    • A. Split a polychromatic beam of radiation into its component wavelengths
    • B. Selectively filter certain wavelengths from a beam of I.R. radiation
    • C. Modulate the I.R. signal at a lower frequency, so that it can be observed by a detector
    • D. none of the above
    • Discuss
    • 9. When radiation energy is absorbed by a spin 1/2 nucleus in a magnetic field, what happens?

    • Options
    • A. The processional frequency of the nucleus increases
    • B. The nucleus spins faster
    • C. The angle of precession flips so that the magnetic moment of the nucleus opposes the applied field
    • D. none of the above
    • Discuss
    • 10. An FT-IR instrument record a signal in the

    • Options
    • A. time domain
    • B. frequency domain
    • C. both (a) and (b)
    • D. none of these
    • Discuss


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