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Home Biochemistry FT IR Spectroscopy Comments

  • Question
  • How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field?


  • Options
  • A. 2
  • B. 4
  • C. 3
  • D. 6

  • Correct Answer



  • FT IR Spectroscopy problems


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    • 1. In scanning electron microscopy

    • Options
    • A. a specimen is fixed and then coated with thin layer of a heavy metal
    • B. a specimen is fixed and then coated with transition metal
    • C. a specimen is not fixed and then coated with thin layer of a heavy metal
    • D. none of the above
    • Discuss
    • 2. Negative staining is a technique used in

    • Options
    • A. electron microscopy
    • B. gel electrophoresis
    • C. immunocytochemistry
    • D. light microscopy
    • Discuss
    • 3. How do you turn a signal recorded in the time domain into a frequency domain signal?

    • Options
    • A. Fourier transformation
    • B. Measurement of peak areas
    • C. By use of a Michelson interferometer
    • D. None of the above
    • Discuss
    • 4. Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis?

    • Options
    • A. Molecular mass
    • B. Solubility
    • C. Isoelectric point
    • D. Folding
    • Discuss
    • 5. Cytochrome C has an isoelectric pH of

    • Options
    • A. 8.5
    • B. 10.05
    • C. 7.5
    • D. 11.05
    • Discuss
    • 6. In FT-NMR, how are nuclei excited?

    • Options
    • A. By radio-frequency radiation whose frequency is swept across a predetermined range
    • B. By an intense pulse of radiation which contains a wide range of frequencies
    • C. By an intense pressure
    • D. None of the above
    • Discuss
    • 7. In immunofluroscence microscopy, fluroscent compounds are attached to

    • Options
    • A. an antibody specific for the subcellular structure
    • B. an antigen specific for the subcellular structure
    • C. bound antibody specific for the subcellular structure
    • D. none of these
    • Discuss
    • 8. What is meant by rotating frame of reference?

    • Options
    • A. That the sample is spun rapidly in the applied field
    • B. If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space
    • C. That the detector rotates around the sample
    • D. None of the above
    • Discuss
    • 9. The sequence of amino acids in proteins can be determined by means of

    • Options
    • A. identification of the -NH2 terminal amino acids
    • B. identification of the -COOH terminal amino acids
    • C. partial cleavage of the original polypeptide into smaller polypeptides
    • D. all of the above
    • Discuss
    • 10. The frequency of precession, the transition frequency and the Larmor frequency are

    • Options
    • A. different terms for the same frequency
    • B. same terms for the same frequency
    • C. different terms for the different frequency
    • D. same terms for the different frequency
    • Discuss


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