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- Question
Negative staining is a technique used in
Options- A. electron microscopy
- B. gel electrophoresis
- C. immunocytochemistry
- D. light microscopy
- Correct Answer
- electron microscopy
- 1. How do you turn a signal recorded in the time domain into a frequency domain signal?
Options- A. Fourier transformation
- B. Measurement of peak areas
- C. By use of a Michelson interferometer
- D. None of the above Discuss
- 2. Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis?
Options- A. Molecular mass
- B. Solubility
- C. Isoelectric point
- D. Folding Discuss
- 3. Cytochrome C has an isoelectric pH of
Options- A. 8.5
- B. 10.05
- C. 7.5
- D. 11.05 Discuss
- 4. When placed in a magnetic field, all the random spins of the nuclei
Options- A. stop
- B. reverse direction
- C. align with the magnetic field
- D. rotate to 90° away from the induced field Discuss
- 5. Why is it important to use a deuterated solvent?
Options- A. NMR uses least of this solvent
- B. So the spectrometer can lock onto the sample to prevent the spectrum from drifting during aquisition
- C. Expensive solvents work best with NMR
- D. They dissolve polymer the fastest Discuss
- 6. In scanning electron microscopy
Options- A. a specimen is fixed and then coated with thin layer of a heavy metal
- B. a specimen is fixed and then coated with transition metal
- C. a specimen is not fixed and then coated with thin layer of a heavy metal
- D. none of the above Discuss
- 7. How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field?
Options- A. 2
- B. 4
- C. 3
- D. 6 Discuss
- 8. In FT-NMR, how are nuclei excited?
Options- A. By radio-frequency radiation whose frequency is swept across a predetermined range
- B. By an intense pulse of radiation which contains a wide range of frequencies
- C. By an intense pressure
- D. None of the above Discuss
- 9. In immunofluroscence microscopy, fluroscent compounds are attached to
Options- A. an antibody specific for the subcellular structure
- B. an antigen specific for the subcellular structure
- C. bound antibody specific for the subcellular structure
- D. none of these Discuss
- 10. What is meant by rotating frame of reference?
Options- A. That the sample is spun rapidly in the applied field
- B. If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space
- C. That the detector rotates around the sample
- D. None of the above Discuss
FT IR Spectroscopy problems
Search Results
Correct Answer: Fourier transformation
Correct Answer: Isoelectric point
Correct Answer: 10.05
Correct Answer: align with the magnetic field
Correct Answer: So the spectrometer can lock onto the sample to prevent the spectrum from drifting during aquisition
Correct Answer: a specimen is fixed and then coated with thin layer of a heavy metal
Correct Answer: 2
Correct Answer: By radio-frequency radiation whose frequency is swept across a predetermined range
Correct Answer: an antibody specific for the subcellular structure
Correct Answer: If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space
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