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Home Biochemistry NMR Spectroscopy Comments

  • Question
  • Coupling causes the peaks in 1H NMR spectra to be split into


  • Options
  • A. two peaks
  • B. multiple peaks equal to the number of hydrogens on surrounding atoms
  • C. multiple peaks equal to the number of surrounding carbon atoms
  • D. multiple peaks equal to the number of hydrogen on surrounding atoms, plus one

  • Correct Answer
  • multiple peaks equal to the number of hydrogen on surrounding atoms, plus one 


  • NMR Spectroscopy problems


    Search Results


    • 1. Better understanding of the nuclei is possible,

    • Options
    • A. with the help of wavelength spectrum
    • B. with the help of frequencies ranges
    • C. with the help of a mathematical translator called the fourier transfer algorithm
    • D. none of the above
    • Discuss
    • 2. What is used to cool the superconducting coil?

    • Options
    • A. Hydrogen
    • B. Ice
    • C. Dry ice
    • D. Liquid helium
    • Discuss
    • 3. What is shielding in NMR?

    • Options
    • A. Using a curved piece of metal to block an opponents attack
    • B. Putting metal around an Rf source
    • C. When the magnetic moment of an atom blocks the full induced magnetic field from surrounding nuclei
    • D. Blocking parts of a molecule from Rf radiation
    • Discuss
    • 4. All hydrogen atoms

    • Options
    • A. have the same resonance frequency
    • B. resonate at different frequencies depending on their environment.
    • C. are attached to carbon
    • D. resonate at about the same frequency as carbon
    • Discuss
    • 5. Which of the following statement is correct?

    • Options
    • A. Sodium and chloride are the chief extracellular ions of the body
    • B. Sodium and chloride are primarily involved in maintaining osmotic equilibrium and body fluid volume.
    • C. Chloride ion is necessary for the production of hydrochloric acid of gastric juice.
    • D. All of these
    • Discuss
    • 6. Why is it important to use a deuterated solvent?

    • Options
    • A. NMR uses least of this solvent
    • B. So the spectrometer can lock onto the sample to prevent the spectrum from drifting during aquisition
    • C. Expensive solvents work best with NMR
    • D. They dissolve polymer the fastest
    • Discuss
    • 7. When placed in a magnetic field, all the random spins of the nuclei

    • Options
    • A. stop
    • B. reverse direction
    • C. align with the magnetic field
    • D. rotate to 90° away from the induced field
    • Discuss
    • 8. Cytochrome C has an isoelectric pH of

    • Options
    • A. 8.5
    • B. 10.05
    • C. 7.5
    • D. 11.05
    • Discuss
    • 9. Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis?

    • Options
    • A. Molecular mass
    • B. Solubility
    • C. Isoelectric point
    • D. Folding
    • Discuss
    • 10. How do you turn a signal recorded in the time domain into a frequency domain signal?

    • Options
    • A. Fourier transformation
    • B. Measurement of peak areas
    • C. By use of a Michelson interferometer
    • D. None of the above
    • Discuss


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