logo

CuriousTab

CuriousTab

Discussion


Home Biochemistry Recombinant DNA Technology Comments

  • Question
  • Which of the following techniques can be used to determine the defective gene and for developing cancer?


  • Options
  • A. Western blot
  • B. Southern blot
  • C. Northern blot
  • D. Eastern blot

  • Correct Answer
  • Southern blot 


  • Recombinant DNA Technology problems


    Search Results


    • 1. In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel.

    • Options
    • A. negative ... positive
    • B. basic ... acidic
    • C. long ... short
    • D. positive to negative
    • Discuss
    • 2. In order to insert a foreign gene into a plasmid, both must __________

    • Options
    • A. have identical DNA sequences
    • B. originate from the same type of cell
    • C. be cut by the same restriction enzyme
    • D. be of the same length
    • Discuss
    • 3. Plant transformation can be obtained by

    • Options
    • A. combining plant and animal cells in culture
    • B. shooting DNA into plant cells with a gun
    • C. using the E. coli bacterium to infect plant roots
    • D. infecting plants with a tobacco mosaic virus
    • Discuss
    • 4. Which of the following is correct in terms of determination of location of genetic traits?

    • Options
    • A. Known protein coding sequences are too far apart to allow linkage determination for most new genes
    • B. Restriction sites allow DNAs to be digested
    • C. Protein-coding genes are always associated with a restriction pattern
    • D. None of the above
    • Discuss
    • 5. An expression vector

    • Options
    • A. always contains an origin of replication
    • B. usually contains a gene that confers antibiotic resistance to the bacterial host
    • C. always contains DNA segments for the regulation of mRNA production
    • D. all of the above
    • Discuss
    • 6. Isoschizomers recognize

    • Options
    • A. same recognition sequence but different recognition site
    • B. same recognition site and recognition sequence
    • C. same recognition site and different recognition sequence
    • D. different recognition site and different recognition sequence
    • Discuss
    • 7. Which of the following genetic diseases would be amenable to genetic engineering?

    • Options
    • A. Down's syndrome
    • B. Muscular dystrophy
    • C. Cystic fibrosis
    • D. Cri du Chat
    • Discuss
    • 8. First discovered, Type II restriction endonuclease was

    • Options
    • A. Hinf I
    • B. Eco K
    • C. Hind II
    • D. EcoRI
    • Discuss
    • 9. Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

    • Options
    • A. copies of the gene with an altered sequence so that a restriction site is inserted
    • B. mutagenized copies of a gene
    • C. short sequences that will hybridize only to a specific base sequence
    • D. PCR-amplified variable numbers of tandem repeats (VNTRs)
    • Discuss
    • 10. Which of the following can be diagnosed by amniocentesis?

    • Options
    • A. Down's syndrome
    • B. Cystic Fibrosis
    • C. Sickle cell anemia
    • D. All of these
    • Discuss


    Comments

    There are no comments.

Enter a new Comment