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Home Biochemistry Recombinant DNA Technology Comments

  • Question
  • In order to insert a foreign gene into a plasmid, both must __________


  • Options
  • A. have identical DNA sequences
  • B. originate from the same type of cell
  • C. be cut by the same restriction enzyme
  • D. be of the same length

  • Correct Answer
  • be cut by the same restriction enzyme 


  • Recombinant DNA Technology problems


    Search Results


    • 1. Plant transformation can be obtained by

    • Options
    • A. combining plant and animal cells in culture
    • B. shooting DNA into plant cells with a gun
    • C. using the E. coli bacterium to infect plant roots
    • D. infecting plants with a tobacco mosaic virus
    • Discuss
    • 2. Which of the following is correct in terms of determination of location of genetic traits?

    • Options
    • A. Known protein coding sequences are too far apart to allow linkage determination for most new genes
    • B. Restriction sites allow DNAs to be digested
    • C. Protein-coding genes are always associated with a restriction pattern
    • D. None of the above
    • Discuss
    • 3. An expression vector

    • Options
    • A. always contains an origin of replication
    • B. usually contains a gene that confers antibiotic resistance to the bacterial host
    • C. always contains DNA segments for the regulation of mRNA production
    • D. all of the above
    • Discuss
    • 4. An example of a restriction fragment length polymorphism is

    • Options
    • A. an Eco RI cuts DNA at a different sequence than Hind III
    • B. different length fragments of DNA resulting from loss or gain of a restriction site
    • C. cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
    • D. all of the above
    • Discuss
    • 5. The TP53 gene of chromosome 17 codes for a protein __________ .

    • Options
    • A. that plays a role in the digestive process
    • B. involved in glucose transport
    • C. involved in the regulation of the cell cycle
    • D. that is like a white blood cell protein
    • Discuss
    • 6. In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel.

    • Options
    • A. negative ... positive
    • B. basic ... acidic
    • C. long ... short
    • D. positive to negative
    • Discuss
    • 7. Which of the following techniques can be used to determine the defective gene and for developing cancer?

    • Options
    • A. Western blot
    • B. Southern blot
    • C. Northern blot
    • D. Eastern blot
    • Discuss
    • 8. Isoschizomers recognize

    • Options
    • A. same recognition sequence but different recognition site
    • B. same recognition site and recognition sequence
    • C. same recognition site and different recognition sequence
    • D. different recognition site and different recognition sequence
    • Discuss
    • 9. Which of the following genetic diseases would be amenable to genetic engineering?

    • Options
    • A. Down's syndrome
    • B. Muscular dystrophy
    • C. Cystic fibrosis
    • D. Cri du Chat
    • Discuss
    • 10. First discovered, Type II restriction endonuclease was

    • Options
    • A. Hinf I
    • B. Eco K
    • C. Hind II
    • D. EcoRI
    • Discuss


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