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  • Question
  • Thin layer chromatography is


  • Options
  • A. partition chromatography
  • B. electrical mobility of ionic species
  • C. adsorption chromatography
  • D. none of the above

  • Correct Answer
  • adsorption chromatography 


  • Chromatography problems


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    • 1. The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)

    • Options
    • A. V = V0 + kDVi
    • B. V = V0/Vi
    • C. V = V0 - kDVi
    • D. V/V0 = kDVi
    • Discuss
    • 2. In reverse phase chromatography, the stationary phase is made

    • Options
    • A. non-polar
    • B. polar
    • C. either non-polar or polar
    • D. none of these
    • Discuss
    • 3. Ion exchange chromatography is based on the

    • Options
    • A. electrostatic attraction
    • B. electrical mobility of ionic species
    • C. adsorption chromatography
    • D. partition chromatography
    • Discuss
    • 4. In gas chromatography, the basis for separation of the components of the volatile material is the difference in

    • Options
    • A. partition coefficients
    • B. conductivity
    • C. molecular weight
    • D. molarity
    • Discuss
    • 5. Internal conversion is where

    • Options
    • A. the spin of an excited electron reverses, changing the state of the molecule (from singlet state to triplet state or vice versa)
    • B. A molecule converts its excess energy to light, and emits a photon
    • C. A molecule converts excess electronic energy to vibrational energy
    • D. none of the above
    • Discuss
    • 6. A combination of paper chromatography and electrophoresis involves

    • Options
    • A. partition chromatography
    • B. electrical mobility of the ionic species
    • C. both (a) and (b)
    • D. none of these
    • Discuss
    • 7. Which of the following statements is true for a refractive index detector in HPLC?

    • Options
    • A. It is more sensitive than a UV detector
    • B. It can only be used for isocratic elutions
    • C. It does not respond to many solutes
    • D. none of above
    • Discuss
    • 8. Which can be used as a mobile phase in HPLC applications?

    • Options
    • A. Any compound with solubility in liquid
    • B. Any compound with limited solubility in liquid
    • C. Any compound with non-solubility in liquid
    • D. Any of the above
    • Discuss
    • 9. Void volume refers to the

    • Options
    • A. total volume of eluent in the column the remainder being taken up by the packing material
    • B. the volume of solvent contained in a liquid chromatographic column
    • C. the time required for the gradient to reach the column
    • D. the volume of the column between the point at which solvents are mixed and the beginning of the column
    • Discuss
    • 10. HPLC stands for

    • Options
    • A. High Pressure Liquid Chromatography
    • B. High Performance Liquid Chromatography
    • C. both (a) and (b)
    • D. Highly Placed Liquid Chromatography
    • Discuss


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